MicroRNA-21 accelerates hepatocyte proliferation in vitro via PI3K/Akt signaling by targeting PTEN

被引：77

作者：

Bai Yan-nan ^{[1
,2
]}

Yu Zhao-yan ^{[1
]}

Luo Li-xi ^{[1
]}

Yi Jiang ^{[1
]}

Xia Qing-jie ^{[3
]}

Zeng Yong ^{[1
]}

机构：

[1] Sichuan Univ, West China Hosp, Dept Hepatobiliary Pancreat Surg, Chengdu 610041, Sichuan Provinc, Peoples R China

[2] Fujian Med Univ, Prov Clin Coll, Fujian Prov Hosp, Dept Hepatobiliary Surg, Fuzhou 350001, Fujian Province, Peoples R China

[3] Sichuan Univ, West China Med Sch, West China Hosp, Translat Neurosci Ctr, Chengdu 610041, Sichuan Provinc, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 443卷 / 03期

关键词：

Liver regeneration; Proliferation; microRNA-21; Phosphatase and tensin homologue deleted on chromosome 10; CELL-CYCLE PROGRESSION; LIVER-REGENERATION; PARTIAL-HEPATECTOMY; PROTEIN-KINASE; EXPRESSION; PHASE; CANCER; GENE; ACTIVATION; MECHANISM;

D O I：

10.1016/j.bbrc.2013.12.047

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

MicroRNAs (miRNAs) are involved in controlling hepatocyte proliferation during liver regeneration. In this study, we established the miRNAs-expression patterns of primary hepatocytes in vitro under stimulation of epidermal growth factor (EGF), and found that microRNA-21 (miR-21) was appreciably up-regulated and peaked at 12 h. In addition, we further presented evidences indicating that miR-21 promotes primary hepatocyte proliferation through in vitro transfecting with miR-21 mimics or inhibitor. We further demonstrated that phosphatidylinositol 3'-OH kinase (PI3K)/Akt signaling was altered accordingly, it is, by targeting phosphatase and tensin homologue deleted on chromosome 10, PI3K/Akt signaling is activated by miR-21 to accelerate hepatocyte rapid S-phase entry and proliferation in vitro. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：802 / 807

页数：6

共 38 条

[1]

Involvement of miR-21 in resistance to daunorubicin by regulating PTEN expression in the leukaemia K562 cell line [J].