Extraction of salinomycin from finished layers ration by microwave solvent extraction followed by liquid chromatography

A rapid, reliable, environmentally friendly and economical extraction technique was developed for the extraction of sodium salinomycin (referred to as salinomycin antibiotic to prevent coccidiosis in broilers) in finished feeds, The method involved a microwave extraction procedure using an ordinary microwave oven, Feed mixed with ethanol-propan-2-ol (15 + 2) was irradiated with microwave energy for 8 s, Salinomycin was detected by HPLC at 592 nm after post-column derivatization with dimethylaminobenzaldehyde at 85 degrees C, The salinomycin concentration determined in finished feeds was 34, 60, 103 and 160 mg kg(-1), compared with calculated values of 30, 60, 90 and 150 mg kg(-1), respectively, The corresponding values obtained with the conventional technique were 27, 45, 59 and 112 mg kg(-1). Salinomycin is stable in finished feeds for more than 8 months.