Polygalacturonase-inhibiting protein (PGIP) from Japanese pear: possible involvement in resistance against scab

被引：26

作者：

Faize, M ^{[1
]}

Sugiyama, T ^{[1
]}

Faize, L ^{[1
]}

Ishii, H ^{[1
]}

机构：

[1] Natl Inst Agroenvironm Sci, Ibaraki 3058604, Japan

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2003年 / 63卷 / 06期

基金：

日本学术振兴会;

关键词：

polygalacturonase-inhibiting protein; nucleotide sequence; pear scab; Venturia nashicola; Japanese pear; European pear;

D O I：

10.1016/j.pmpp.2004.03.006

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Venturia nashicola is the causal agent of scab, a fungal disease affecting Asian pears. The Japanese pear cv. 'Kousui' is highly susceptible to the race I of this fungus whereas the cv. 'Kinchaku' and the non-host European pear cv. 'Flemish Beauty' are resistant. The aim of this work is to investigate the role of polygalacturonase-inhibiting proteins (PGIPs) of pear during the interactions with V. nashicola leading to susceptibility or resistance. PGIP protein was detected from immature fruit of Kousui and Kinchaku. It showed a molecular mass of 42 kDa that shifted to 35 kDa after chemical deglycosylation. The gene pgip was amplified by PCR using genomic DNA and/or cDNA from young leaves of Kousui, Kinchaku, and European pear cvs. Flemish Beauty, 'Bartlett', and an Asian wild pear strain 'Mamenashi 12', then sequenced after sub-cloning. Some conserved variations were identified in the sequence indicating that gene family also exists in pgip of Japanese pear and confirmed by Southern blot analysis. The expression of PGIP was studied in scab-inoculated leaves of the susceptible Kousui and the resistant Kinchaku and Flemish Beauty. pgip Gene and its encoding protein were highly and rapidly activated in these resistant plants. In addition, PGIP extracts derived from Kinchaku and Flemish Beauty partially inhibited the activity of polygalacturonase (PG) from V nashicola suggesting a possible role of PGIP in limiting fungal growth frequently observed in these resistant cultivars. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：319 / 327

页数：9

共 33 条

[21]

MULLER M, 1993, DEV PLANT PATHOL, V2, P68

[22] Functional Characterization of Citrus Polygalacturonase-inhibiting Protein [J].