Resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance

被引:76
作者
Brotman, Y
Silberstein, L
Kovalski, I
Perin, C
Dogimont, C
Pitrat, M
Klingler, J
Thompson, GA
Perl-Treves, R [1 ]
机构
[1] Bar Ilan Univ, Fac Life Sci, IL-52900 Ramat Gan, Israel
[2] INRA, Stn Genet & Ameliorat Fruit & Legumes, F-84143 Montfavet, France
[3] Univ Arizona, Dept Plant Sci, Tucson, AZ 85721 USA
[4] Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA
关键词
melon; Cucumis melo; R-genes; resistance gene homologues; NBS-LRR; RFLP; Aphis gossypii; Fusarium oxysporum;
D O I
10.1007/s00122-001-0808-x
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Genomic and cDNA fragments with homology to known disease resistance genes (RGH fragments) were cloned from Cucumis melo using degenerate-primer PCR. Fifteen homologues of the NBS-LRR gene family have been isolated. The NBS-LRR homologues show high divergence and, based on the partial NBS-fragment sequences, appear to include members of the two major subfamilies that have been described in dicot plants, one that possesses a TIR-protein element and one that lacks such a domain. Genomic organization of these sequences was explored by DNA gel-blot analysis, and conservation among other Cucurbitaceae was assessed. Two mapping populations that segregate for several disease and pest resistance loci were used to map the RGH probes onto the melon genetic map. Several NBS-LRR related sequences mapped to the vicinity of genetic loci that control resistance to papaya ringspot virus, Fusarium oxysporian race 1, F oxysporum race 2 and to the insect pest Aphis gossypii. The utility of such markers for breeding resistant melon cultivars and for cloning the respective R-genes is discussed.
引用
收藏
页码:1055 / 1063
页数:9
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