Pharmacological activation changes stiffness of cultured human airway smooth muscle cells

被引:95
作者
Hubmayr, RD
Shore, SA
Fredberg, JJ
Planus, E
Panettieri, RA
Moller, W
Heyder, J
Wang, N
机构
[1] HARVARD UNIV, SCH PUBL HLTH, DEPT ENVIRONM HLTH, PHYSIOL PROGRAM, BOSTON, MA 02115 USA
[2] UNIV PENN, DEPT MED, DIV PULM & CRIT CARE, PHILADELPHIA, PA 19102 USA
[3] GSF, NATL RES CTR ENVIRONM & HLTH, INST INHALAT BIOL, D-85764 OBERSCHLEISSHEIM, GERMANY
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1996年 / 271卷 / 05期
关键词
magnetic twisting cytometry; cell mechanics; cytoskeleton; signal transduction; smooth muscle contraction;
D O I
10.1152/ajpcell.1996.271.5.C1660
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Using magnetic twisting cytometry (MTC), we measured the cytoskeletal stiffness of adherent human airway smooth muscle (HASM) cells. We hypothesized that modulation of actin-myosin interactions by application of contractile agonists would induce changes in cytoskeletal stiffness. In cells plated on high-density collagen, bradykinin (10(-6) M) and histamine (10(-4) M) increased stiffness by 85 +/- 15 and 68 +/- 16%, respectively. Increases in cell stiffness were also consistently observed after acetylcholine, substance P, and KCl. The bronchodilator agonists isoproterenol, prostaglandin E(2), forskolin, dibutryl adenosine 3',5'-cyclic monophosphate, and 8-bromoguanosine S',5'-cyclic monophosphate each caused a dose-dependent decrease in cell stiffness in unstimulated as well as bradykinin-treated cells. HASM cells plated on high-density collagen were stiffer than cells plated on low-density collagen (126 +/- 16 vs. 43 +/- 3 dyn/cm(2)) and developed more pronounced increases in stiffness in response to bradykinin as well as more pronounced decreases in stiffness in response to isoproterenol. These results are consistent with the hypothesis that modulation of actin-myosin interactions by application of contractile agonists causes changes in cytoskeletal stiffness of HASM cells. MTC may be a valuable tool for evaluating the mechanisms of pharmacomechanical coupling in ail-way smooth muscle cells in culture.
引用
收藏
页码:C1660 / C1668
页数:9
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