Laparoscopic splenectomy and nephrectomy in a rat model - Description of a new technique

被引：11

作者：

Giuffrida, MC ^{[1
]}

Marquet, RL ^{[1
]}

Kazemier, G ^{[1
]}

Wittich, P ^{[1
]}

Bouvy, ND ^{[1
]}

Bruining, HA ^{[1
]}

Bonjer, HJ ^{[1
]}

机构：

[1] UNIV HOSP DIJKZIGT,DEPT SURG,NL-3015 GD ROTTERDAM,NETHERLANDS

来源：

SURGICAL ENDOSCOPY-ULTRASOUND AND INTERVENTIONAL TECHNIQUES | 1997年 / 11卷 / 05期

关键词：

laparoscopy; rat; model; splenectomy; nephrectomy; tumor;

D O I：

10.1007/s004649900400

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Background: In experimental studies on the effects of laparoscopic procedures on tumor biology, a localized tumor model is desirable. The spleen and the kidney are preferable, because these organs are amenable to tumor placement and subsequent removal. This study describes the technique of laparoscopic splenectomy and nephrectomy in the rat model. Methods: Pneumoperitoneum was established by CO2 insufflation. Laparoscopic splenectomy involved two-handed dissection, intracorporeal ligation, and division of gastrosplenic attachments and hilar and short gastric vessels. Laparoscopic nephrectomy was done by intracorporeal ligation and division of the renal vessels and the ureter after mobilization of the kidney. Results: Laparoscopic splenectomy was performed in six rats; laparoscopic nephrectomy was done in six rats. Operative time ranged from 45 to 90 min for splenectomy and from 40 to 65 min for nephrectomy. Postoperatively, two rats died from hemorrhage. Necropsy of the rats after 10 days revealed adhesion in three rats after splenectomy and in four rats after nephrectomy. Inflammatory processes were found around the silk ligatures in all rats after splenectomy; in two rats wound infections occurred at the port sites. Conclusions: Laparoscopic splenectomy and nephrectomy in the rat proved technically feasible and may provide new localized tumor models suitable to be used in further studies on the oncological effects of laparoscopic surgery.

引用

页码：491 / 494

页数：4

共 18 条

[1]

ALLENDORF JDF, 1995, ARCH SURG-CHICAGO, V130, P649

[2] LAPAROSCOPIC COLON SURGERY IN A RAT MODEL - A PRELIMINARY-REPORT [J].

BERGUER, R ;

GUTT, CN .

SURGICAL ENDOSCOPY-ULTRASOUND AND INTERVENTIONAL TECHNIQUES, 1994, 8 (10) :1195-1197

[3] LAPAROSCOPIC SURGERY IN THE RAT - DESCRIPTION OF A NEW TECHNIQUE [J].

BERGUER, R ;

GUTT, C ;

STIEGMANN, GV .

SURGICAL ENDOSCOPY-ULTRASOUND AND INTERVENTIONAL TECHNIQUES, 1993, 7 (04) :345-347

[4] PREDICTIVE TESTING WITH THE SUBRENAL CAPSULE ASSAY [J].

BOGDEN, AE ;

GRIFFIN, W ;

REICH, SD ;

COSTANZA, ME ;

COBB, WR .

CANCER TREATMENT REVIEWS, 1984, 11 :113-124

[5]

BOHM B, 1993, SURG ENDOSC-ULTRAS, V8, P707

[6] Laparoscopic surgery in the rat - Beneficial effect on body weight and tumor take [J].

Bouvy, ND ;

Marquet, RL ;

Hamming, JF ;

Jeekel, J ;

Bonjer, HJ .

SURGICAL ENDOSCOPY-ULTRASOUND AND INTERVENTIONAL TECHNIQUES, 1996, 10 (05) :490-494

[7]

BOUVY ND, 1994, EUR SURG RES, V26, P75

[8]

BOUVY ND, 1997, IN PRESS BR J SURG

[9] FROM LAPAROSCOPIC TRAINING ON AN ANIMAL-MODEL TO RETROPERITONEOSCOPIC OR CELIOSCOPIC ADRENAL AND RENAL SURGERY IN HUMAN [J].

DECANNIERE, L ;

LORGE, F ;

ROSIERE, A ;

JOUCKEN, K ;

MICHEL, LA .

SURGICAL ENDOSCOPY-ULTRASOUND AND INTERVENTIONAL TECHNIQUES, 1995, 9 (06) :699-701

[10]

DUGUE L, 1995, ANN CHIR, V49, P423

← 1 2 →