Heterogeneity of Entamoeba histolytica rac genes encoding p21(rac) homologues

被引：35

作者：

Lohia, A ^{[1
]}

Samuelson, J ^{[1
]}

机构：

[1] HARVARD UNIV,SCH PUBL HLTH,DEPT TROP PUBL HLTH,BOSTON,MA 02115

来源：

GENE | 1996年 / 173卷 / 02期

关键词：

amebic dysentery; GTP-binding; proteins; phagocytosis; polymerase chain reaction; protozoan parasite; rac family proteins;

D O I：

10.1016/0378-1119(96)00232-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Entamoeba histolytica (Eh), the parasite that causes amebic dysentery, is the only protozoan that phagocytoses bacteria, epithelial cells and red blood cells. Numerous low-molecular-weight GTP-binding proteins, called p21(rac), are implicated in signal transduction and actin polymerization during phagocytosis by macrophages and Dictyostelium discoideum (Dd). Here, molecular cloning techniques were used to obtain four Eh rac genes that encoded putative p21(rac), as well as segments of two Eh rac pseudogenes. The predicted Eh p21(rac), which share 55-81% amino acid (aa) identities with each other, include one that closely resembles the p21(rac1) of man, Dd, Drosophila melanogaster and Caenorhabditis elegans; two that resemble the p21(racC) of Dd; and one that is unique. An alignment of the Eh rac ORF with other rac family proteins reveals multiple aa that distinguish p21(rac1), p21(racC) and p21(cdc42). We conclude that the Eh genes encoding amebic p21(rac), which are the first identified from a protozoan parasite, are numerous and heterogeneous.

引用

页码：205 / 208

页数：4

共 21 条

[11] RHO, RAC, AND CDC42 GTPASES REGULATE THE ASSEMBLY OF MULTIMOLECULAR FOCAL COMPLEXES ASSOCIATED WITH ACTIN STRESS FIBERS, LAMELLIPODIA, AND FILOPODIA [J].

NOBES, CD ;

HALL, A .

CELL, 1995, 81 (01) :53-62

[12] AMEBIASIS [J].

RAVDIN, JI .

CLINICAL INFECTIOUS DISEASES, 1995, 20 (06) :1453-1464

[13] THE SMALL GTP-BINDING PROTEIN RAC REGULATES GROWTH-FACTOR INDUCED MEMBRANE RUFFLING [J].

RIDLEY, AJ ;

PATERSON, HF ;

JOHNSTON, CL ;

DIEKMANN, D ;

HALL, A .

CELL, 1992, 70 (03) :401-410

[14] THE SMALL GTP-BINDING PROTEIN RHO REGULATES THE ASSEMBLY OF FOCAL ADHESIONS AND ACTIN STRESS FIBERS IN RESPONSE TO GROWTH-FACTORS [J].

RIDLEY, AJ ;

HALL, A .

CELL, 1992, 70 (03) :389-399

[15] ULTRASTRUCTURAL-LOCALIZATION OF RAS-RELATED PROTEINS USING EPITOPE-TAGGED PLASMIDS [J].

ROBERTSON, D ;

PATERSON, HF ;

ADAMSON, P ;

HALL, A ;

MONAGHAN, P .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1995, 43 (05) :471-480

[16]

SELF AJ, 1993, ONCOGENE, V8, P655

[17] MOLECULAR-CLONING OF RAS AND RAP GENES FROM ENTAMOEBA-HISTOLYTICA [J].

SHEN, PS ;

LOHIA, A ;

SAMUELSON, J .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1994, 64 (01) :111-120

[18] MOLECULAR-CLONING OF THE GENE FOR THE HUMAN PLACENTAL GTP-BINDING PROTEIN-GP (G25K) - IDENTIFICATION OF THIS GTP-BINDING PROTEIN AS THE HUMAN HOMOLOG OF THE YEAST CELL-DIVISION-CYCLE PROTEIN-CDC42 [J].

SHINJO, K ;

KOLAND, JG ;

HART, MJ ;

NARASIMHAN, V ;

JOHNSON, DI ;

EVANS, T ;

CERIONE, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (24) :9853-9857

[19] PATTERN-INDUCED MULTI-SEQUENCE ALIGNMENT (PIMA) ALGORITHM EMPLOYING SECONDARY STRUCTURE-DEPENDENT GAP PENALTIES FOR USE IN COMPARATIVE PROTEIN MODELING [J].

SMITH, RF ;

SMITH, TF .

PROTEIN ENGINEERING, 1992, 5 (01) :35-41

[20] THE RAS PROTEIN FAMILY - EVOLUTIONARY TREE AND ROLE OF CONSERVED AMINO-ACIDS [J].

VALENCIA, A ;

CHARDIN, P ;

WITTINGHOFER, A ;

SANDER, C .

BIOCHEMISTRY, 1991, 30 (19) :4637-4648

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