Replication fork regression in repetitive DNAs

被引：74

作者：

Fouche, Nicole ^{[1
]}

Ozgur, Sezgin ^{[1
]}

Roy, Debasmita ^{[1
]}

Griffith, Jack D. ^{[1
]}

机构：

[1] Univ N Carolina, Dept Biochem & Biophys, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA

来源：

NUCLEIC ACIDS RESEARCH | 2006年 / 34卷 / 20期

关键词：

D O I：

10.1093/nar/gkl757

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Among several different types of repetitive sequences found in the human genome, this study has examined the telomeric repeat, necessary for the protection of chromosome termini, and the disease-associated triplet repeat (CTG).(CAG)(n). Evidence suggests that replication of both types of repeats is problematic and that a contributing factor is the repetitive nature of the DNA itself. Here we have used electron microscopy to investigate DNA structures formed at replication forks on large model DNAs containing these repeat sequences, in an attempt to elucidate the contributory effect that these repetitive DNAs may have on their replication. Visualization of the DNA revealed that there is a high propensity for a paused replication fork to spontaneously regress when moving through repetitive DNAs, and that this results in a four-way chickenfoot intermediate that could present a significant block to replication in vivo, possibly leading to unwanted recombination events, amplifications or deletions.

引用

页码：6044 / 6050

页数：7

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