Selective nanoscale positioning of ferritin and nanoparticles by means of target-specific peptides

被引：65

作者：

Yamashita, Khiro

Kirimura, Hiroya

Okuda, Mitsuhiro

Nishio, Kazuaki

Sano, Ken-Ichi

Shiba, Kiyotaka

Hayashi, Tomohiro

Hara, Masahiko

Mishima, Yumiko

机构：

[1] Matsushita Elect Ind Co Ltd, Adv Technol Res Labs, Seika, Kyoto 6190237, Japan

[2] Japanese Fdn Canc Res, Koto Ku, Tokyo 1358550, Japan

[3] JST, CREST, Koto Ku, Tokyo 1358550, Japan

[4] Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Midori Ku, Yokohama, Kanagawa 2268502, Japan

[5] RIKEN, Frontier Res Syst, Local Spatiotemporal Funct Lab, Wako, Saitama 3510198, Japan

[6] JST, CREST, Kawaguchi, Saitama 3320012, Japan

来源：

SMALL | 2006年 / 2卷 / 10期

关键词：

biomineralization; nanomanipulation; nanoparticles; protein structures; surface modification;

D O I：

10.1002/smll.200600220

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The synthesis of nanometer-sized titanium (Ti) surfaces by nanosphere lithography and selective nanoscale positioning of ferritin by means of target specific peptides is analyzed. Ferittin is a ubiquitous iron-storage protein and has a spherical hollow shell composed of 24 subunits and ferrihydride core. A study is conducted to modify ferittin surface so that a specific surface can be discerned and ferittin selectively absorbs on it. A tri-bonding peptide motif with a high affinity for Titanium (Ti) is used in the study. The atomic structure of the coreless minTBP1 peptide is analyzed by X-ray crystallography. The X-ray study reveals that the Ti-binding motif is mobile, confirming that the motif has the steric freedom required to form an appropriate structure with in an affinity for Ti. The adsorption of the iron oxide cores is observed by field-emission scanning electron microscopy. The experiment can be used to place many kinds of Nampa onto a substrate.

引用

页码：1148 / 1152

页数：5

共 26 条

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