Difference in quasispecies of the hypervariable region 1 of hepatitis C virus between alcoholic and non-alcoholic patients

被引：28

作者：

Takahashi, K ^{[1
]}

Takahashi, T ^{[1
]}

Takahashi, S ^{[1
]}

Watanabe, K ^{[1
]}

Boku, S ^{[1
]}

Matsui, S ^{[1
]}

Arai, F ^{[1
]}

Asakura, H ^{[1
]}

机构：

[1] Niigata Univ, Sch Med, Dept Internal Med 3, Niigata 9518122, Japan

来源：

JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY | 2001年 / 16卷 / 04期

关键词：

chronic liver disease; habitual alcohol intake; hepatitis C virus; polymerase chain; reaction-single strand conformation polymorphism; quasispecies;

D O I：

10.1046/j.1440-1746.2001.02462.x

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background: Habitual alcohol intake is known to aggravate the clinical outcome of hepatitis C virus (HCV)-related chronic liver diseases and to increase the risk of hepatocellular carcinoma. Methods: To investigate the possible mechanism of these effects by alcohol, we examined 31 cases of HCV-related chronic liver diseases of which 17 cases were drinking just before admission and the remaining 14 cases were non-drinkers. The studied cases included 18 patients with chronic hepatitis, six with liver cirrhosis and seven with hepatocellular carcinoma. The quasispecies of the hypervariable region 1 of the HCV genome were analyzed by using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Hepatitis C virus viral load was quantitated by using multicyclic PCR after reverse transcription of the 5 non-coding region of the genome. Results: The mean PCR-SSCP band number that reflected the quasispecies complexity in hypervariable region 1 was more significantly increased in alcoholics than in non-alcoholics (5.5 +/- 1.4 vs 3.9 +/- 1.1, P < 0.01). The significant increase in alcoholics remained, even if the cases were restricted to males (P < 0.01), to HCV genotype 1b (P < 0.05) or to chronic hepatitis (P < 0.05). The HCV viral load was not statistically different between alcoholic and non-alcoholic HCV-related chronic liver diseases (5.02 x 10(6) +/- 5.16 x 10(6) copies/mL vs 9.00 x 10(7) +/- 2.75 x 10(8) copies/mL, P = 0.28). Mutation events seemed to occur randomly when amino acid sequences of hypervariable region 1 were compared between four drinkers and four non-drinkers. Conclusions: The enhanced quasispecies complexity in hypervariable region 1 of HCV in alcoholics may be the main cause of more progressive HCV-related chronic liver diseases, and may provide the disease the resistance against any therapeutic modalities including interferon. (C) 2001 Blackwell Science Asia Pty Ltd.

引用

页码：416 / 423

页数：8

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