Non-derivatized analysis of methiin and alliin in vegetables by capillary electrophoresis

被引:14
作者
Horie, Hideki [1 ]
Yamashita, Ken-Ichiro [1 ]
机构
[1] Natl Agr & Food Res Org, Natl Inst Vegetable & Tea Sci, Tsu, Mie 5142392, Japan
关键词
Allium; Brassica; S-alk(en)yl cysteine sulfoxide; flavor; Chinese chive; garlic;
D O I
10.1016/j.chroma.2006.09.018
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Methiin and alliin are important components of flavors or the precursors of flavors and odors of Allium vegetables. Moreover, they are thought to be beneficial to health. A non-derivative method was developed to analyze these compounds in vegetables by capillary electrophoresis. These compounds in the extracts of Allium and Brassica vegetables were detected indirectly at 225 nm. The analysis of each sample required less than 25 min, and the linear detection range was 5-5000 mg/l. This method was simple compared to the other published methods using high performance liquid chromatography. Moreover, it was possible to detect the peak of pyruvate simultaneously with methiin and alliin using this method. The presence of pyruvate peak is a useful indicator if the blanching of the samples has been insufficient. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:337 / 339
页数:3
相关论文
共 15 条
[1]   High-performance ion-pair chromatography method for simultaneous analysis of alliin, deoxyalliin, allicin and dipeptide precursors in garlic products using multiple mass spectrometry and UV detection [J].
Arnault, I ;
Christidès, JP ;
Mandon, N ;
Haffner, T ;
Kahane, R ;
Auger, J .
JOURNAL OF CHROMATOGRAPHY A, 2003, 991 (01) :69-75
[2]   SURVEY OF VOLATILE COMPONENTS OF SOME ALLIUM SPECIES IN TERMS OF S-ALK(EN)YL-L-CYSTEINE SULFOXIDES PRESENT AS FLAVOR PRECURSORS [J].
FREEMAN, GG ;
WHENHAM, RJ .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1975, 26 (12) :1869-1886
[3]  
HORIE H, 2006, B NATL I VEG TEA SCI, V5, P1
[4]  
Hosoda H, 2005, J JPN SOC FOOD SCI, V52, P120, DOI 10.3136/nskkk.52.120
[5]   THERMAL-DEGRADATION PRODUCTS OF S-ALKYL-L-CYSTEINE OCCURRING IN THE ALLIUM-SPECIES WITH D-GLUCOSE [J].
KIMURA, K ;
IWATA, I ;
NISHIMURA, H ;
KASUYA, M ;
YAMANE, A ;
MIZUTANI, J .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1990, 54 (08) :1893-1903
[6]   Gas chromatographic determination of S-alk(en)ylcysteine sulfoxides [J].
Kubec, R ;
Svobodová, M ;
Velísek, J .
JOURNAL OF CHROMATOGRAPHY A, 1999, 862 (01) :85-94
[7]   Thermal degradation of S-methylcysteine and its sulfoxide -: Important flavor precursors of Brassica and Allium vegetables [J].
Kubec, R ;
Drhová, V ;
Velísek, J .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (10) :4334-4340
[8]   Antibacterial activity of heated cabbage juice, S-methyl-L-cysteine sulfoxide and methyl methanethiosulfonate [J].
Kyung, KH ;
Han, DC ;
Fleming, HP .
JOURNAL OF FOOD SCIENCE, 1997, 62 (02) :406-409
[9]   The analysis of onion and garlic [J].
Lanzotti, V .
JOURNAL OF CHROMATOGRAPHY A, 2006, 1112 (1-2) :3-22
[10]   SIMPLE METHOD FOR APPROXIMATE DETERMINATION OF S-METHYLCYSTEINE SULFOXIDE (KALE ANEMIA FACTOR) [J].
MATHESON, NA ;
MOIR, AW .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1976, 27 (10) :959-961