Genotyping faecal samples of Bengal tiger Panthera tigris tigris for population estimation:: A pilot study

被引：84

作者：

Bhagavatula, Jyotsna ^{[1
]}

Singh, Lalji ^{[1
]}

机构：

[1] Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India

来源：

BMC GENETICS | 2006年 / 7卷 / 1期

关键词：

D O I：

10.1186/1471-2156-7-48

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Background: Bengal tiger Panthera tigris tigris the National Animal of India, is an endangered species. Estimating populations for such species is the main objective for designing conservation measures and for evaluating those that are already in place. Due to the tiger's cryptic and secretive behaviour, it is not possible to enumerate and monitor its populations through direct observations; instead indirect methods have always been used for studying tigers in the wild. DNA methods based on non-invasive sampling have not been attempted so far for tiger population studies in India. We describe here a pilot study using DNA extracted from faecal samples of tigers for the purpose of population estimation. Results: In this study, PCR primers were developed based on tiger-specific variations in the mitochondrial cytochrome b for reliably identifying tiger faecal samples from those of sympatric carnivores. Microsatellite markers were developed for the identification of individual tigers with a sibling Probability of Identity of 0.005 that can distinguish even closely related individuals with 99.9% certainty. The effectiveness of using field-collected tiger faecal samples for DNA analysis was evaluated by sampling, identification and subsequently genotyping samples from two protected areas in southern India. Conclusion: Our results demonstrate the feasibility of using tiger faecal matter as a potential source of DNA for population estimation of tigers in protected areas in India in addition to the methods currently in use.

引用

页数：12

共 49 条

[11]

Franklin Neil, 1999, P130

[12] Reliable microsatellite genotyping of the Eurasian badger (Meles meles) using faecal DNA [J].

Frantz, AC ;

Pope, LC ;

Carpenter, PJ ;

Roper, TJ ;

Wilson, GJ ;

Delahay, RJ ;

Burke, T .

MOLECULAR ECOLOGY, 2003, 12 (06) :1649-1661

[13] Empirical evaluation of preservation methods for faecal DNA [J].

Frantzen, MAJ ;

Silk, JB ;

Ferguson, JWH ;

Wayne, RK ;

Kohn, MH .

MOLECULAR ECOLOGY, 1998, 7 (10) :1423-1428

[14]

GAGNEUX P, 1997, MOL ECOL, V6, P862

[15] A multi-samples, multi-extracts approach for microsatellite analysis of faecal samples in an arboreal ape [J].

Goossens B. ;

Chikhi L. ;

Utami S.S. ;

De Ruiter J. ;

Bruford M.W. .

Conservation Genetics, 2000, 1 (2) :157-162

[16] Estimation of tiger densities in the tropical dry forests of Panna, Central India, using photographic capture-recapture sampling [J].

Karanth, KU ;

Chundawat, RS ;

Nichol, JD ;

Kumar, NS .

ANIMAL CONSERVATION, 2004, 7 :285-290

[17] Science deficiency in conservation practice: the monitoring of tiger populations in India [J].

Karanth, KU ;

Nichols, JD ;

Seidensticker, J ;

Dinerstein, E ;

Smith, JLD ;

McDougal, C ;

Johnsingh, AJT ;

Chundawat, RS ;

Thapar, V .

ANIMAL CONSERVATION, 2003, 6 :141-146

[18]

Karanth KU, 1998, ECOLOGY, V79, P2852

[19] Conservation status of tigers in a primary rainforest of Peninsular Malaysia [J].

Kawanishi, K ;

Sunquist, ME .

BIOLOGICAL CONSERVATION, 2004, 120 (03) :329-344

[20] Estimating population size by genotyping faeces [J].

Kohn, MH ;

York, EC ;

Kamradt, DA ;

Haugt, G ;

Sauvajot, RM ;

Wayne, RK .

PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1999, 266 (1420) :657-663

← 1 2 3 4 5 →