Three-dimensional characterization of drug-encapsulating particles using STEM detector in FEG-SEM

被引：11

作者：

Barkay, Zahava ^{[1
]}

Rivkin, Ilia ^{[2
]}

Margalit, Rimona ^{[2
]}

机构：

[1] Tel Aviv Univ, Wolfson Appl Mat Res Ctr, IL-69978 Tel Aviv, Israel

[2] Tel Aviv Univ, Dept Biochem, Fac Life Sci, IL-69978 Tel Aviv, Israel

来源：

MICRON | 2009年 / 40卷 / 04期

关键词：

Low energy STEM; SEM; Mass-thickness contrast; Drug-encapsulating particles; SCANNING-ELECTRON-MICROSCOPY; LOW-ENERGY; WET-STEM; SCATTERING; RESOLUTION;

D O I：

10.1016/j.micron.2008.12.003

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

New drug-encapsulating particles were investigated using bright field (BF) scanning transmission electron Microscopy (STEM) in a field emission gun (FEG) scanning electron microscope (SEM). Thickness characterization was done based on measuring the effective cross-section for interaction in our sample-detector configuration using calibration particles. A simplified analytical model, taking account of BF-STEM contrast and effective cross-section for interaction, was utilized for transforming projected two-dimensional BF-STEM images into three-dimensional thickness images. The three-dimensional characterization is demonstrated on a new family of biological materials composed of submicron to Micron drug-free and drug-encapsulating particles. The importance of using BF-STEM in SEM, relative to other electron microscopy methods, is discussed as well as the lateral and depth resolution. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：480 / 485

页数：6

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