Development of a fluorescent intercalator displacement assay (G4-FID) for establishing quadruplex-DNA affinity and selectivity of putative ligands

被引：201

作者：

Monchaud, David ^{[1
]}

Allain, Clemence ^{[1
]}

Teulade-Fichou, Marie-Paule ^{[1
]}

机构：

[1] CNRS, Coll France, UPR 285, Lab Chim Interact Mol, F-75005 Paris, France

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2006年 / 16卷 / 18期

关键词：

G-quadruplex; thiazole orange; fluorescent intercalator displacement;

D O I：

10.1016/j.bmcl.2006.06.067

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A fluorescent intercalator displacement assay (G4-FID) has been designed based on the displacement of thiazole orange (TO) positioned onto a quadruplex-forming oligonucleotide by putative ligands. This technique was validated by the use of a set of representative and fully characterized G-quadruplex binders (ranging from pyridodicarboxamide to macrocyclic ligands). To further extend its applicability, a comparative version has been developed which allows a rapid and viable determination of quadruplex-over duplex-selectivity. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：4842 / 4845

页数：4

共 29 条

[1]

ALLAIN C, UNPUB

[2] INTERACTION OF ETHIDIUM WITH SYNTHETIC DOUBLE-STRANDED POLYNUCLEOTIDES AT LOW IONIC-STRENGTH [J].

BAGULEY, BC ;

FALKENHAUG, EM .

NUCLEIC ACIDS RESEARCH, 1978, 5 (01) :161-171

[3] Total synthesis of distamycin A and 2640 analogues: A solution-phase combinatorial approach to the discovery of new, bioactive DNA binding agents and development of a rapid, high-throughput screen for determining relative DNA binding affinity or DNA binding sequence selectivity [J].

Boger, DL ;

Fink, BE ;

Hedrick, MP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (27) :6382-6394

[4] A simple, high-resolution method for establishing DNA binding affinity and sequence selectivity [J].

Boger, DL ;

Fink, BE ;

Brunette, SR ;

Tse, WC ;

Hedrick, MP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (25) :5878-5891

[5] MICROGONOTROPENS AND THEIR INTERACTIONS WITH DNA .2. QUANTITATIVE-EVALUATION OF EQUILIBRIUM-CONSTANTS FOR 1/1 AND 2/1 BINDING OF DIEN-MICROGONOTROPEN-A, DIEN-MICROGONTROPEN-B, AND DIEN-MICROGONTROPEN-C AS WELL AS DISTAMYCIN AND HOECHST-33258 TO D(GGCGCAAATTTGGCGG)/D(CCGCCAATTTGCGCC) [J].

BROWNE, KA ;

HE, GX ;

BRUICE, TC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (16) :7072-7079

[6]

Gavathiotis E, 2001, ANGEW CHEM INT EDIT, V40, P4749, DOI 10.1002/1521-3773(20011217)40:24<4749::AID-ANIE4749>3.0.CO

[7]

2-I

[8] Interaction of telomestatin with the telomeric single-strand overhang [J].

Gomez, D ;

Paterski, R ;

Lemarteleur, T ;

Shin-ya, K ;

Mergny, JL ;

Riou, JF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (40) :41487-41494

[9] Preferential binding of a G-quadruplex ligand to human chromosome ends [J].

Granotier, C ;

Pennarun, G ;

Riou, L ;

Hoffschir, F ;

Gauthier, LR ;

De Cian, A ;

Gomez, D ;

Mandine, E ;

Riou, JF ;

Mergny, JL ;

Mailliet, P ;

Dutrillaux, B ;

Boussin, FD .

NUCLEIC ACIDS RESEARCH, 2005, 33 (13) :4182-4190

[10] High-resolution assessment of protein DNA binding affinity and selectivity utilizing a fluorescent intercalator displacement (FID) assay [J].

Ham, YW ;

Tse, WC ;

Boger, DL .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2003, 13 (21) :3805-3807

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