Chromate-induced human erythrocytes haemoglobin oxidation and peroxidation:: influence of vitamin E, vitamin C, salicylate, deferoxamine, and N-ethylmaleimide

被引：20

作者：

Fernandes, MAS ^{[1
]}

Geraldes, CFGC

Oliveira, CR

Alpoim, MC

机构：

[1] Univ Coimbra, Fac Ciencias, Dept Zool, P-3004517 Coimbra, Portugal

[2] Univ Coimbra, Fac Ciencias, Dept Bioquim, P-3001401 Coimbra, Portugal

[3] Univ Coimbra, Fac Med, Serv Bioquim, P-3004504 Coimbra, Portugal

来源：

TOXICOLOGY LETTERS | 2000年 / 114卷 / 1-3期

关键词：

antioxidants; chromate; cytotoxicity; haemoglobin oxidation; human erythrocytes; oxidative stress;

D O I：

10.1016/S0378-4274(00)00167-3

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

In order to attenuate or to prevent chromate-induced human erythrocytes injury, the influence of Vitamin E, vitamin C, salicylate, deferoxamine, and N-ethylmaleimide on chromate-induced human erythrocytes haemoglobin oxidation and peroxidation were investigated. It was observed that pretreatment of human erythrocytes with vitamin E (20 mu M), vitamin C (1 mM), salicylate (3 mM), and deferoxamine (4 mM) significantly increased (P = 0.0001) chromate-induced human erythrocytes haemoglobin oxidation in a time dependent manner, while it was significantly decreased (P = 0.0001) by pretreatment with N-ethylmaleimide (1 mM). In contrast, pretreatment of human erythrocytes with deferoxamine (4 mM) immediately inhibited (P = 0.0001) chromate-induced human erythrocytes peroxidation, while it was significantly increased (P = 0.0001) by pretreatment with N-ethylmaleimide (1 mM) during the first 4 h of cells exposition to chromate. For time periods superior to 6 h pretreatment with N-ethylmaleimide (1 mM) significantly decreased (P = 0.0001) chromate-induced human erythrocytes peroxidation. It was concluded that care must be taken as these drugs are used to prevent against toxicity induced by chromium(VI) compounds. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：237 / 243

页数：7

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