H-1 NMR analysis of novel sialylated and fucosylated lactose-based oligosaccharides having linear GlcNAc(beta 1-6) Gal and Neu5Ac(alpha 2-6) GlcNAc sequences

被引：4

作者：

Bailey, D

Davies, MJ

Routier, FH

Bauer, C

Feeney, J

Hounsell, EF

机构：

[1] UNIV LONDON UNIV COLL,DEPT BIOCHEM & MOL BIOL,FUNCT GRP,MRC GLYCOPROT STRUCT,LONDON WC1E 6BT,ENGLAND

[2] NATL INST MED RES,MRC,BIOMED NMR CTR,LONDON NW7 1AA,ENGLAND

来源：

CARBOHYDRATE RESEARCH | 1997年 / 300卷 / 04期

关键词：

NMR spectroscopy; milk oligosaccharides; gastrointestinal tract; breast fed infants;

D O I：

10.1016/S0008-6215(96)00338-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three novel oligosaccharides of human infant faeces have been fully characterised by methylation analysis and 500/600 MHz H-1 NMR spectroscopy including DQF-COSY, TQF-COSY, TOCSY and ROESY experiments. The oligosaccharides were shown to be lactose-based structures two of which were substituted at C-6 of Gal with either the Le(x) trisaccharide, Gal(beta 1-4)[Fuc(alpha 1-3)]GlcNAc(beta 1-, or Neu5Ac(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-. They differ from other free oligosaccharides previously isolated from the human by having the (1 --> 6) linkage to Gal in the absence of a (1 --> 3) branch. The third oligosaccharide has Neu5Ac(alpha 2-6) linked to GlcNAc of the trisaccharide GlcNAc(beta 1-3)Gal(beta 1-4)Glc. This is a linear fragment of the disialylated tetrasaccharide sequence Neu5Ac(alpha 2-3)Gal( beta 1-3)[Neu5Ac(alpha 2-6)]GlcNAc(beta 1- found in the milk oligosaccharide disialyl LNT (the GlcNAc residue of the tetrasaccharide linked to lactose) and also of N-linked chains (GlcNAc linked to Man). (C) 1997 Elsevier Science Ltd.

引用

页码：289 / 300

页数：12

共 44 条

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