Stir bar sorptive extraction-LC-MS for the analysis of fluoxetine in plasma

被引：40

作者：

Fernandes, C.

Jiayu, P.

Sandra, P.

Lancas, F. M.

机构：

[1] Univ Fed Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP, Brazil

[2] Univ Ghent, Dept Organ Chem, B-9000 Ghent, Belgium

来源：

CHROMATOGRAPHIA | 2006年 / 64卷 / 9-10期

基金：

巴西圣保罗研究基金会;

关键词：

column liquid chromatography-mass spectrometry; stir bar sorptive extraction; antidepressant drug; fluoxetine in human plasma;

D O I：

10.1365/s10337-006-0078-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, sensitive, and selective method for the analysis of fluoxetine in plasma samples is described in this study. Stir bar sorptive extraction combined with liquid chromatography and mass spectrometry has been successfully used in this procedure. Plasma samples were first submitted to protein precipitation with trichloroacetic acid, subjected to SBSE, and thereafter analyzed by LC-MS. The method presented specificity, linearity over the concentration range of 10-500 ng mL(-1), precision, and adequate sensitivity for the determination of fluoxetine in plasma.

引用

页码：517 / 521

页数：5

共 32 条

[1] Determination of fluoxetine and norfluoxetine in plasma by a modified high performance reversed phase liquid chromatographic method with fluorescence detection [J].