Pasteurella multocida exbB, exbD and tonB genes are physically linked but independently transcribed

被引：37

作者：

Bosch, M

Garrido, E

Llagostera, M

de Rozas, AMP

Badiola, I ^{[1
]}

Barbé, J

机构：

[1] Univ Autonoma Barcelona, Dept Gen Microbiol, Bellaterra 08193, Spain

[2] UAB IRTA, CReSA, Bellaterra 08193, Spain

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 210卷 / 02期

关键词：

exbB gene; exbD gene; tonB gene; iron regulation; overlapping gene; Pasteurella multocida;

D O I：

10.1111/j.1574-6968.2002.tb11181.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The exbB, exbD and tonB genes of the Pasteurella multocida animal pathogen have been cloned by complementation of an Escherichia coli tonB mutant. Despite these three genes being physically linked, RT-PCR analysis, lacZ transcriptional fusions and construction of insertional mutants have demonstrated that they do not constitute an operon, but rather are transcribed independently from each other. Futhermore, expression of these three genes is under iron control as revealed by lacZ fusions and Fur titration assay analysis. Moreover, each of these genes is necessary for the virulence of P. multocida cells and all of them contribute equally to the infectious process of this microorganism.

引用

页码：201 / 208

页数：8

共 25 条

[11] Promoter identification and expression analysis of Salmonella typhimurium and Escherichia coli nrdEF operons encoding one of two class I ribonucleotide reductases present in both bacteria [J].