Bidirectional Regulation of mRNATranslation in Mammalian Cells by Using PUF Domains

被引：36

作者：

Cao, Jicong ^{[1
]}

Arha, Manish ^{[1
]}

Sudrik, Chaitanya ^{[1
]}

Schaffer, David V. ^{[2
]}

Kane, Ravi S. ^{[1
]}

机构：

[1] Rensselaer Polytech Inst, Dept Chem & Biol Engn, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA

[2] Univ Calif Berkeley, Dept Chem Engn, Helen Wills Neurosci Inst, Calif Inst Quantitat Biosci, Berkeley, CA 94720 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2014年 / 53卷 / 19期

关键词：

gene expression; gene technology; inducible gene expression; optogenetics; RNA; EUKARYOTIC GENE-EXPRESSION; PUMILIO-HOMOLOGY DOMAIN; MESSENGER-RNA; TRANSLATIONAL REGULATION; PROTEIN INTERACTIONS; LIVING CELLS; INITIATION; BINDING; RECOGNITION; ACTIVATION;

D O I：

10.1002/anie.201402095

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The regulation of gene expression is crucial in diverse areas of biological science, engineering, and medicine. A genetically encoded system based on the RNA binding domain of the Pumilio and FBF (PUF) proteins was developed for the bidirectional regulation (i.e., either upregulation or downregulation) of the translation of a target mRNA. PUF domains serve as designable scaffolds for the recognition of specific RNA elements and the specificity can be easily altered to target any 8-nucleotide RNA sequence. The expression of a reporter could be varied by over 17-fold when using PUF-based activators and repressors. The specificity of the method was established by using wild-type and mutant PUF domains. Furthermore, this method could be used to activate the translation of target mRNA downstream of PUF binding sites in a light-dependent manner. Such specific bidirectional control of mRNA translation could be particularly useful in the fields of synthetic biology, developmental biology, and metabolic engineering.

引用

页码：4900 / 4904

页数：5

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