Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824

被引:158
作者
Green, EM
Boynton, ZL
Harris, LM
Rudolph, FB
Papoutsakis, ET
Bennett, GN
机构
[1] RICE UNIV, INST BIOSCI & BIOENGN, DEPT BIOCHEM & CELL BIOL, HOUSTON, TX 77005 USA
[2] NORTHWESTERN UNIV, DEPT CHEM ENGN, EVANSTON, IL 60208 USA
来源
MICROBIOLOGY-SGM | 1996年 / 142卷
关键词
Clostridium acetobutylicum; solvent biosynthesis; acid biosynthesis; metabolic engineering; gene integration;
D O I
10.1099/13500872-142-8-2079
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Integrational plasmid technology has been used to disrupt metabolic pathways leading to acetate and butyrate formation in Clostridium acetobutylicum ATCC 824. Non-replicative plasmid constructs, either clostridial phosphotransacetylase (pta) or butyrate kinase (buk) gene fragments, were integrated into homologous regions on the chromosome. Integration was assumed to occur by a Campbell-like mechanism, inactivating either pta or buk. Inactivation of the pta gene reduced phosphotransacetylase and acetate kinase activity and significantly decreased acetate production. Inactivation of the buk gene reduced butyrate kinase activity, significantly decreased butyrate production and increased butanol production.
引用
收藏
页码:2079 / 2086
页数:8
相关论文
共 36 条
[31]  
WALTER KA, 1993, THESIS NW U EVANSTON
[32]   PHOSPHOTRANSBUTYRYLASE FROM CLOSTRIDIUM-ACETOBUTYLICUM ATCC-824 AND ITS ROLE IN ACIDOGENESIS [J].
WIESENBORN, DP ;
RUDOLPH, FB ;
PAPOUTSAKIS, ET .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (02) :317-322
[33]   TARGETED INTEGRATION OF GENES INTO THE CLOSTRIDIUM-ACETOBUTYLICUM CHROMOSOME [J].
WILKINSON, SR ;
YOUNG, M .
MICROBIOLOGY-SGM, 1994, 140 :89-95
[34]   RECENT ADVANCES IN THE GENETICS OF THE CLOSTRIDIA [J].
YOUNG, M ;
MINTON, NP ;
STAUDENBAUER, WL .
FEMS MICROBIOLOGY LETTERS, 1989, 63 (04) :301-325
[35]  
YOUNG M, 1984, J GEN MICROBIOL, V130, P1613
[36]   STABILITY OF REITERATED SEQUENCES IN THE BACILLUS-SUBTILIS CHROMOSOME [J].
YOUNG, M ;
EHRLICH, SD .
JOURNAL OF BACTERIOLOGY, 1989, 171 (05) :2653-2656