Removal of common Fusarium toxins in vitro by strains of Lactobacillus and Propionibacterium

被引:250
作者
El-Nezami, HS
Chrevatidis, A
Auriola, S
Salminen, S
Mykkänen, H
机构
[1] Univ Kuopio, Dept Clin Nutr, FIN-70211 Kuopio, Finland
[2] Univ Kuopio, Food & Hlth Res Ctr, FIN-70211 Kuopio, Finland
[3] Univ Kuopio, Dept Pharmaceut Chem, FIN-70211 Kuopio, Finland
[4] Univ Turku, Dept Biochem & Food Chem, FIN-20014 Turku, Finland
来源
FOOD ADDITIVES AND CONTAMINANTS | 2002年 / 19卷 / 07期
关键词
trichothecenes; mycotoxin; lactic acid bacteria; toxin binding;
D O I
10.1080/02652030210134236
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
This study was conducted to examine the ability of selected strains of Lactobacillus and Propionibacterium to remove common Fusarium toxins, trichothecenes, from liquid media. The trichothecenes studied were deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-AcDON), nivalenol (NIV), fusarenon (FX), diacetoxyscirpenol (DAS), T-2 toxin (T-2) and HT-2 toxin (HT-2). The Lactobacillus rhamnosus strain GG (LGG), Lactobacillus rhamnosus strain LC-705 (LC-705) and Propionibacterium freudenreichii ssp. shermanii JS (PJS) were incubated in PBS buffer containing 20 mug toxin ml(-1) for 1 h at 37degreesC, and after centrifugation the concentration of the toxins was measured in the supernatant fraction. Both viable and heat-killed forms of LGG and PJS were more efficient than LC-705 in removing the toxins from the liquid media. LGG and PJS removed four of the seven tested toxins (the removal varying from 18 to 93%) and LC-705 two toxins (10-64%). Of the toxins, 3-AcDON was not removed by any of the bacteria; HT-2 was removed by the non-viable LGG and also slightly by non-viable LC-705; DAS was removed by all three bacteria tested. Binding is postulated as the possible mechanism of the removal, since no difference was observed between the ability of viable and heat-killed bacteria in removing the trichothecenes, and no degradation products of the toxins were detected by gas chromatography (GC) mass spectrometry (MS) analysis. It is concluded that significant differences exist in the ability of the bacteria to bind trichothecenes in vitro.
引用
收藏
页码:680 / 686
页数:7
相关论文
共 24 条
  • [1] BIOTRANSFORMATION AND DETOXIFICATION OF T-2 TOXIN BY SOIL AND FRESH-WATER BACTERIA
    BEETON, S
    BULL, AT
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (01) : 190 - 197
  • [2] Cole R.J., 1981, HDB TOXIC FUNGAL MET
  • [3] EXCRETION OF DEOXYNIVALENOL AND ITS METABOLITE IN MILK, URINE, AND FECES OF LACTATING DAIRY-COWS
    COTE, LM
    DAHLEM, AM
    YOSHIZAWA, T
    SWANSON, SP
    BUCK, WB
    [J]. JOURNAL OF DAIRY SCIENCE, 1986, 69 (09) : 2416 - 2423
  • [4] Physicochemical alterations enhance the ability of dairy strains of lactic acid bacteria to remove aflatoxin from contaminated media
    El-Nezami, H
    Kankaanpaa, P
    Salminen, S
    Ahokas, J
    [J]. JOURNAL OF FOOD PROTECTION, 1998, 61 (04) : 466 - 468
  • [5] Ability of dairy strains of lactic acid bacteria to bind a common food carcinogen, aflatoxin B1
    El-Nezami, H
    Kankaanpaa, P
    Salminen, S
    Ahokas, J
    [J]. FOOD AND CHEMICAL TOXICOLOGY, 1998, 36 (04) : 321 - 326
  • [6] Ability of Lactobacillus and Propionibacterium strains to remove aflatoxin B1 from the chicken duodenum
    El-Nezami, H
    Mykkänen, H
    Kankaanpää, P
    Salminen, S
    Ahokas, J
    [J]. JOURNAL OF FOOD PROTECTION, 2000, 63 (04) : 549 - 552
  • [7] El-Nezami H., 2000, Bioscience and Microflora, V19, P41
  • [8] Mycotoxins: Their implications for human and animal health
    Fink-Gremmels, J
    [J]. VETERINARY QUARTERLY, 1999, 21 (04) : 115 - 120
  • [9] Factors affecting the sequestration of aflatoxin by Lactobacillus rhamnosus strain GG
    Haskard, C
    Binnion, C
    Ahokas, J
    [J]. CHEMICO-BIOLOGICAL INTERACTIONS, 2000, 128 (01) : 39 - 49
  • [10] Surface binding of aflatoxin B1 by lactic acid bacteria
    Haskard, CA
    El-Nezami, HS
    Kankaanpää, PE
    Salminen, S
    Ahokas, JT
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (07) : 3086 - 3091