The effect of interspecific oocytes on demethylation of sperm DNA

被引：101

作者：

Beaujean, N

Taylor, JE

McGarry, M

Gardner, JO

Wilmut, I

Loi, P

Ptak, G

Galli, C

Lazzari, G

Bird, A

Young, LE ^{[1
]}

Meehan, RR

机构：

[1] Roslin Inst, Div Gene Express & Dev, Roslin EH25 9PS, Midlothian, Scotland

[2] Univ Edinburgh, Dept Biomed & Clin Lab Sci, Edinburgh EH8 9XD, Midlothian, Scotland

[3] Teramo Univ, Dept Comparat Biomed Sci, I-64100 Teramo, Italy

[4] CIZ srl, Lab Tecnol Riproduz, Ist Sperimentale Italiano Lazzaro Spallazani, I-26100 Cremona, Italy

[5] Univ Edinburgh, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland

[6] Univ Nottingham, Inst Genet, Queens Med Ctr, Nottingham NG7 2UH, England

[7] Univ Nottingham, Div Obstet & Gynecol, Queens Med Ctr, Nottingham NG7 2UH, England

[8] Western Gen Hosp, Human Genome Unit, MRC, Edinburgh EH4 9XU, Midlothian, Scotland

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2004年 / 101卷 / 20期

关键词：

D O I：

10.1073/pnas.0400730101

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In contrast to mice, in sheep no genome-wide demethylation of the paternal genome occurs within the first postfertilization cell cycle. This difference could be due either to an absence of a sheep demethylase activity that is present in mouse ooplasm or to an increased protection of methylated cytosine residues in sheep sperm. Here, we use interspecies intracytoplasmic sperm injection to demonstrate that sheep sperm DNA can be demethylated in mouse oocytes. Surprisingly, mouse sperm can also be demethylated to a limited extent in sheep oocytes. Our results suggest that the murine demethylation process is facilitated either by a sperm-derived factor or by male pronuclear chromatin composition.

引用

页码：7636 / 7640

页数：5

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[1] Genome-wide methylation patterns in normal and uniparental early mouse embryos [J].