Aberrant uterine natural killer (NK)-cell expression and altered placental and serum levels of the NK-cell promoting cytokine interleukin-12 in pre-eclampsia

被引:93
作者
Bachmayer, Nora
Hamad, Rangeen Rafik
Liszka, Lucie
Bremme, Katarina
Sverremark-Ekstrom, Eva
机构
[1] Stockholm Univ, Wenner Gren Inst, Arrhenius Labs Nat Sci F5, Dept Immunol, S-10691 Stockholm, Sweden
[2] Karolinska Univ Hosp, Karolinska Inst, Dept Women & Child Hlth, Div Obstet & Gynecol, Stockholm, Sweden
关键词
decidual CD56(+) cells; interleukin-10-12-15-18; pre-eclampsia; pregnancy;
D O I
10.1111/j.1600-0897.2006.00429.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Natural killer (NK) cells are the most abundant lymphocyte population at the maternal-fetal interface. They are suggested to be important during placentation by controlling trophoblast invasion. If placentation is suboptimal, pre-eclampsia can occur. Decidual NK (dNK) cells were examined at delivery in 46 women, 22 pre-eclamptic women and 24 healthy controls, by staining for CD56 and CD94 with immunohistochemistry (IHC). Furthermore, we investigated the placental expression and the serum levels of the NK-cell activating cytokines interleukin(IL)-12, IL-15, IL-18 and the anti-inflammatory cytokine IL-10 by IHC and enzyme-linked immunosorbent assay (ELISA), respectively. Pre-eclamptic women had higher number of CD56(+) and CD94(+) cells in the decidua, indicating an altered receptor expression of dNK cells. We also demonstrate for the first time that the villous trophoblasts show strong immunostaining for IL-12 in placentae from healthy controls, while women suffering from pre-eclampsia have significantly less IL-12. However, pre-eclamptic women had significantly elevated IL-12 and IL-15 levels in serum. Results show increased numbers and altered phenotype of dNK cells in pre-eclampsia, supporting the importance of these cells for a healthy pregnancy. The altered receptor expression of dNK cells together with diminished placental IL-12 expression could implicate an altered NK cell-regulation in pre-eclampsia.
引用
收藏
页码:292 / 301
页数:10
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