Development and characterization of methacrylate-based hydrazide monoliths for oriented immobilization of antibodies

被引：19

作者：

Brne, R. ^{[1
]}

Lim, Y. -P ^{[2
]}

Podgornik, A. ^{[1
]}

Barut, M. ^{[1
]}

Pihlar, B. ^{[3
]}

Strancar, A. ^{[1
]}

机构：

[1] BIA Separat doo, Applicat Dept, SI-1000 Ljubljana, Slovenia

[2] ProThera Biol LLC, E Providence, RI 02914 USA

[3] Fac Chem & Chem Technol, Ljubljana 1000, Slovenia

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 13期

关键词：

CIM; Convective interaction media; Monolith; Affinity chromatography; Hydrazide; Antibody immobilization; ALPHA INHIBITOR PROTEINS; AFFINITY-CHROMATOGRAPHY; CELLULOSE; CLEAVAGE; LIGANDS; DESIGN; TOOLS; PHASE;

D O I：

10.1016/j.chroma.2008.11.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Convective interaction media (CIM; BIA Separations) monoliths are attractive stationary phases for use in affinity chromatography because they enable fast affinity binding. which is a consequence of convectively enhanced mass transport. This work focuses on the development of novel CIM hydrazide (HZ) monoliths for the oriented immobilization of antibodies. Adipic acid dihydrazide (AADH) was covalently bound to CIM epoxy monoliths to gain hydrazide groups on the monolith surface. Two different antibodies were afterwards immobilized to hydrazide functionalized monolithic columns and prepared columns were tested for their selectivity. One column was further tested for the dynamic binding capacity. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.

引用

页码：2658 / 2663

页数：6

共 35 条

[1]

Amatschek K, 2000, HRC-J HIGH RES CHROM, V23, P47

[2] Inter-α inhibitor proteins in infants and decreased levels in neonatal sepsis [J].

Baek, YW ;

Brokat, S ;

Padbury, JF ;

Pinar, H ;

Hixson, DC ;

Lim, YP .

JOURNAL OF PEDIATRICS, 2003, 143 (01) :11-15

[3]

Bailon P., 2000, AFFINITY CHROMATOGRA

[4] Monolithic micro-immobilized-enzyme reactor with human recombinant acetylcholinesterase for on-line inhibition studies [J].

Bartolini, M ;

Cavrini, V ;

Andrisano, V .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1031 (1-2) :27-34

[5] Enzyme immobilization on epoxy- and 1,1′-carbonyldiimidazole-activated methacrylate-based monoliths [J].

Bencina, K ;

Podgornik, A ;

Strancar, A ;

Bencina, M .

JOURNAL OF SEPARATION SCIENCE, 2004, 27 (10-11) :811-818

[6]

BENES MJ, 1991, J BIOACT COMPAT POL, V6, P406

[7] Comparison of antibody binding to immobilized group specific affinity ligands in high performance monolith affinity chromatography [J].

Berruex, LG ;

Freitag, R ;

Tennikova, TB .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2000, 24 (01) :95-104

[8] Oriented immobilization of galactose oxidase to bead and magnetic bead cellulose and poly(HEMA-co-EDMA) and magnetic poly(HEMA-co-EDMA) microspheres [J].

Bílková, Z ;

Slováková, M ;

Lycka, A ;

Horák, D ;

Lenfeld, J ;

Turková, J ;

Churácek, J .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 770 (1-2) :25-34

[9] Biomimetic dyes as affinity chromatography tools in enzyme purification [J].

Clonis, YD ;

Labrou, NE ;

Kotsira, VP ;

Mazitsos, C ;

Melissis, S ;

Gogolas, G .

JOURNAL OF CHROMATOGRAPHY A, 2000, 891 (01) :33-44

[10] Affinity chromatography matures as bioinformatic and combinatorial tools develop [J].

Clonis, YD .

JOURNAL OF CHROMATOGRAPHY A, 2006, 1101 (1-2) :1-24

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