Isotope-edited IR spectroscopy for the study of membrane proteins

被引：60

作者：

Arkin, Isaiah T. ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Dept Biol Chem, Alexander Silberman Inst Life Sci, IL-91904 Jerusalem, Israel

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2006年 / 10卷 / 05期

关键词：

D O I：

10.1016/j.cbpa.2006.08.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fourier transform infrared (FTIR) spectroscopy has long been a powerful tool for structural analysis of membrane proteins. However, because of difficulties in resolving contributions from individual residues, most of the derived measurements tend to yield average properties for the system under study. Isotope editing, through its ability to resolve individual vibrations, establishes FTIR as a method that is capable of yielding accurate structural data on individual sites in a protein.

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页码：394 / 401

页数：8

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