Inositol phosphate metabolomics: Merging genetic perturbation with modernized radiolabeling methods

被引：14

作者：

Stevenson-Paulik, Jill ^{[1
]}

Chiou, Shean-Tai ^{[1
]}

Frederick, Josh P. ^{[1
]}

dela Cruz, June ^{[1
]}

Seeds, Andrew M. ^{[1
]}

Otto, James C. ^{[1
]}

York, John D. ^{[1
]}

机构：

[1] Duke Univ, Med Ctr, Howard Hughes Med Inst, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA

来源：

METHODS | 2006年 / 39卷 / 02期

关键词：

high-throughput liquid chromatography; genetic perturbation; systems biology; HPLC; isotopic equilibrium; product proof; extraction; embryonic stem cells; seeds; silique; fly; budding yeast; mouse; human;

D O I：

10.1016/j.ymeth.2006.05.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent discoveries that provide a link between mositol phosphate (IP) signaling and fundamental cellular processes evoke many exciting new hypotheses about IP function, and underscore the importance of understanding how IP synthesis is regulated. Central to studies of IP metabolism is the essential development of efficient, fast, and reproducible methods for quantitative analysis of IP's in systems ranging from simple cell cultures to more complex tissues and whole organisms. Additionally, in many cases there is a need to pharmacologically and/or genetically alter IP kinase and phosphatase activities in order to visualize low abundance inositol signaling messengers. Here, we describe updated methods for rapid analysis of IP metabolism in normal and genetically manipulated Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster, Mus musculus, and Homo sapiens. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：112 / 121

页数：10

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