Phosphatidyl serine exposure during apoptosis precedes release of cytochrome c and decrease in mitochondrial transmembrane potential

被引:91
作者
Denecker, G
Dooms, H
Van Loo, G
Vercammen, D
Grooten, J
Fiers, W
Declercq, W
Vandenabeele, P
机构
[1] Flanders Interuniv Inst Biotechnol, Dept Mol Biol, B-9000 Ghent, Belgium
[2] State Univ Ghent, B-9000 Ghent, Belgium
关键词
apoptosis; phosphatidyl serine; mitochondrial transmembrane potential;
D O I
10.1016/S0014-5793(99)01702-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Time kinetics of phosphatidyl serine (PS) exposure were compared to other apoptotic parameters following different apoptotic stimuli, Our data indicate that anti-Fas treatment of L929sAhFas cells results in rapid exposure of PS, which precedes decrease in mitochondrial transmembrane potential (Delta Psi(m)) and release of cytochrome c, indicating that PS exposure occurs independently of these mitochondrial events. Also during TNF-, etoposide- or staurosporine-mediated apoptosis in PC60 RI/RII cells, PS-positive cells were observed before they had a decreased Delta Psi(m). However, during growth factor depletion-induced death of 32D cells, both phenomena seemed to occur at the same time. (C) 2000 Federation of European Biochemical Societies.
引用
收藏
页码:47 / 52
页数:6
相关论文
共 49 条
  • [11] The role of phosphatidylserine in recognition of apoptotic cells by phagocytes
    Fadok, VA
    Bratton, DL
    Frasch, SC
    Warner, ML
    Henson, PM
    [J]. CELL DEATH AND DIFFERENTIATION, 1998, 5 (07) : 551 - 562
  • [12] Caspases are activated in a branched protease cascade and control distinct downstream processes in Fas-induced apoptosis
    Hirata, H
    Takahashi, A
    Kobayashi, S
    Yonehara, S
    Sawai, H
    Okazaki, T
    Yamamoto, K
    Sasada, M
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 187 (04) : 587 - 600
  • [13] Itano Y, 1996, J NEUROCHEM, V67, P131
  • [14] Effects of differential overexpression of Bcl-2 on apoptosis, proliferation, and telomerase activity in Jurkat T cells
    Johnson, VL
    Cooper, IR
    Jenkins, JR
    Chow, SC
    [J]. EXPERIMENTAL CELL RESEARCH, 1999, 251 (01) : 175 - 184
  • [15] ANNEXIN-V FOR FLOW CYTOMETRIC DETECTION OF PHOSPHATIDYLSERINE EXPRESSION ON B-CELLS UNDERGOING APOPTOSIS
    KOOPMAN, G
    REUTELINGSPERGER, CPM
    KUIJTEN, GAM
    KEEHNEN, RMJ
    PALS, ST
    VANOERS, MHJ
    [J]. BLOOD, 1994, 84 (05) : 1415 - 1420
  • [16] The mitochondrion as an integrator/coordinator of cell death pathways
    Kroemer, G
    [J]. CELL DEATH AND DIFFERENTIATION, 1998, 5 (06) : 547 - 547
  • [17] The mitochondrial death/life regulator in apoptosis and necrosis
    Kroemer, G
    Dallaporta, B
    Resche-Rigon, M
    [J]. ANNUAL REVIEW OF PHYSIOLOGY, 1998, 60 : 619 - 642
  • [18] Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis
    Li, HL
    Zhu, H
    Xu, CJ
    Yuan, JY
    [J]. CELL, 1998, 94 (04) : 491 - 501
  • [19] Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade
    Li, P
    Nijhawan, D
    Budihardjo, I
    Srinivasula, SM
    Ahmad, M
    Alnemri, ES
    Wang, XD
    [J]. CELL, 1997, 91 (04) : 479 - 489
  • [20] Induction of apoptotic program in cell-free extracts: Requirement for dATP and cytochrome c
    Liu, XS
    Kim, CN
    Yang, J
    Jemmerson, R
    Wang, XD
    [J]. CELL, 1996, 86 (01) : 147 - 157