Study on a novel strategy to treatment of phenylketonuria

被引：21

作者：

Liu, JZ ^{[1
]}

Jia, XY ^{[1
]}

Zhang, J ^{[1
]}

Xiang, H ^{[1
]}

Hu, W ^{[1
]}

Zhou, Y ^{[1
]}

机构：

[1] Affiliated Capital Univ Med Sci, Beijing Chaoyang Hosp, Basic Med Res Ctr, Beijing 100020, Peoples R China

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2002年 / 30卷 / 04期

关键词：

phenylalanine amonia lyase; phenylketonuria; hyperphenylalaninemia; intestine; phenylalanine; L; lactis;

D O I：

10.1081/BIO-120006117

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To replace the low phenylalanine (phe) diet treatment and to improve the quality of life of the phenylketonuria (PKU) patients, A phenylalanine ammonia-lyase (PAL) cDNA from petroselinum crispum was subcloned into expression vectors, pMG36e and pNZ8048, and transformed L. lactis by electroporation The pMG36e PAL/L lactis and pNZ8048 PAL/L.L. were screened and characterized by using PCR and HPLC, and prepared as a liquid type preparation that were given orally to treat hyperphenylalaninemia-rats. The phe levels of the rat plasma sample were determined by HPLC. The data showed that the plasma phe levels in hyperphenylalanemia (HPA) rats receiving preparations made from the engineered L.lactis were significantly reduced compared with non-treated HPA rats, The effect of the pNZ8048PAL/L.L. showed a higher expression of PAL and better cure results than pMG36ePAL/L.L. These results point a potential way for PKU treatment.

引用

页码：243 / 257

页数：15

共 15 条

[1] EXTRACORPOREAL ENZYME REACTORS FOR DEPLETION OF PHENYLALANINE IN PHENYLKETONURIA [J].