Protein turnover on the scale of the proteome

被引:56
作者
Doherty, Mary K. [1 ]
Beynon, Robert J. [1 ]
机构
[1] Univ Liverpool, Fac Vet Sci, Prot Funct Grp, Liverpool L69 7ZJ, Merseyside, England
基金
英国生物技术与生命科学研究理事会;
关键词
continuous infusion; flooding dose; mass isotopomer distribution analysis (MIDA); mass spectrometry; protein turnover; proteomics; radioisotopes; stable isotopes;
D O I
10.1586/14789450.3.1.97
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Protein turnover is a neglected dimension in postgenomic studies, defining the dynamics of changes in protein expression and forging a link between transcriptome, proteome and metabolome. Recent advances in postgenomic technologies have led to the development of new proteomic techniques to measure protein turnover on a proteome-wide scale. These methods are driven by stable isotope metabolic labeling of cells in culture or in intact animals. This review considers the merits and difficulties of different methods that allow access to proteome dynamics.
引用
收藏
页码:97 / 110
页数:14
相关论文
共 74 条
  • [31] Integrated genomic and proteomic analyses of a systematically perturbed metabolic network
    Ideker, T
    Thorsson, V
    Ranish, JA
    Christmas, R
    Buhler, J
    Eng, JK
    Bumgarner, R
    Goodlett, DR
    Aebersold, R
    Hood, L
    [J]. SCIENCE, 2001, 292 (5518) : 929 - 934
  • [32] Reevaluation of amino acid stimulation of protein synthesis in murine- and human-derived skeletal muscle cells assessed by independent techniques
    Iresjö, BM
    Svanberg, E
    Lundholm, K
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2005, 288 (05): : E1028 - E1037
  • [33] Johnson HA, 1999, J NUTR, V129, P740, DOI 10.1093/jn/129.3.740
  • [34] A model of whole-body protein turnover based on leucine kinetics in rodents
    Johnson, HA
    Baldwin, RL
    France, J
    Calvert, CC
    [J]. JOURNAL OF NUTRITION, 1999, 129 (03) : 728 - 739
  • [35] The role of protein synthesis and degradation in the post-transcriptional regulation of rat multidrug resistance-associated protein 2 (Mrp2, Abcc2)
    Jones, BR
    Li, W
    Cao, J
    Hoffman, TA
    Gerk, PM
    Vore, M
    [J]. MOLECULAR PHARMACOLOGY, 2005, 68 (03) : 701 - 710
  • [36] SKELETAL-MUSCLE PROTEIN-TURNOVER IN BROILER AND LAYER CHICKS
    JONES, SJ
    ABERLE, ED
    JUDGE, MD
    [J]. JOURNAL OF ANIMAL SCIENCE, 1986, 62 (06) : 1576 - 1583
  • [37] The absolute quantification strategy: a general procedure for the quantification of proteins and post-translational modifications
    Kirkpatrick, DS
    Gerber, SA
    Gygi, SP
    [J]. METHODS, 2005, 35 (03) : 265 - 273
  • [38] Systems biology: A brief overview
    Kitano, H
    [J]. SCIENCE, 2002, 295 (5560) : 1662 - 1664
  • [39] Insights into the relation between mRNA and protein expression patterns:: II.: Experimental observations in Escherichia coli
    Lee, PS
    Shaw, LB
    Choe, LH
    Mehra, A
    Hatzimanikatis, V
    Lee, KH
    [J]. BIOTECHNOLOGY AND BIOENGINEERING, 2003, 84 (07) : 834 - 841
  • [40] A correlation algorithm for the automated quantitative analysis of shotgun proteomics data
    MacCoss, MJ
    Wu, CC
    Liu, HB
    Sadygov, R
    Yates, JR
    [J]. ANALYTICAL CHEMISTRY, 2003, 75 (24) : 6912 - 6921