Distribution of NPM1-ALK and X-ALK fusion transcripts in paediatric anaplastic large cell lymphoma: a molecular-histological correlation

被引：48

作者：

Damm-Welk, Christine ^{[1
]}

Klapper, Wolfram ^{[2
,3
]}

Oschlies, Ilske ^{[2
,3
]}

Gesk, Stefan ^{[4
,5
]}

Roettgers, Silja ^{[1
]}

Bradtke, Jutta ^{[1
]}

Siebert, Reiner ^{[4
,5
]}

Reiter, Alfred ^{[1
]}

Woessmann, Willi ^{[1
]}

机构：

[1] Univ Giessen, Dept Paediat Haematol & Oncol, D-35392 Giessen, Germany

[2] Univ Hosp Schleswig Holstein, Dept Pathol, Haematopathol Sect, Kiel, Germany

[3] Univ Hosp Schleswig Holstein, Lymph Node Registry, Kiel, Germany

[4] Univ Hosp Schleswig Holstein, Inst Human Genet, Kiel, Germany

[5] Univ Kiel, Kiel, Germany

来源：

BRITISH JOURNAL OF HAEMATOLOGY | 2009年 / 146卷 / 03期

关键词：

anaplastic large cell lymphoma; anaplastic lymphoma kinase; NPM-ALK; reverse transcription polymerase chain reaction; paediatric; NPM-ALK; TUMOR;

D O I：

10.1111/j.1365-2141.2009.07754.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

P>Anaplastic large cell lymphomas (ALCL) in children express anaplastic lymphoma kinase (ALK) fusion genes, most commonly NPM1-ALK. The distribution of X-ALK among 66 childhood ALCLs was analysed. One ALCL was ALK-negative. Reverse transcription polymerase chain reaction detected NPM1-ALK in 58 tumours, all showing nuclear and cytoplasmic ALK staining. The remaining seven ALCL stained for ALK in the cytoplasm only: two expressed TPM3-ALK, one ATIC-ALK, one MYH9-ALK; three no TPM3-, TFG-, ATIC-, CLTC- or MYH9-ALK. Almost 90% of paediatric ALK-positive ALCLs express NPM1-ALK. There was complete concordance between ALK staining pattern and the presence of a typical/variant ALK fusion partner.

引用

页码：306 / 309

页数：4

共 13 条

[11] Short-pulse B-non-Hodgkin lymphoma-type chemotherapy is efficacious treatment for pediatric anaplastic large cell lymphoma:: a report of the Berlin-Frankfurt-Munster Group Trial NHL-BFM 90 [J].

Seidemann, K ;

Tiemann, M ;

Schrappe, M ;

Yakisan, E ;

Simonitsch, I ;

Janka-Schaub, G ;

Dörffel, W ;

Zimmermann, M ;

Mann, G ;

Gadner, H ;

Parwaresch, R ;

Riehm, H ;

Reiter, A .

BLOOD, 2001, 97 (12) :3699-3706

[12]

Stein H, 2000, BLOOD, V96, P3681

[13] FISH analysis for the detection of lymphoma-associated chromosomal abnormalities in routine paraffin-embedded tissue [J].

Ventura, Roland A. ;

Martin-Subero, Jose I. ;

Jones, Margaret ;

McParland, Joanna ;

Gesk, Stefan ;

Mason, David Y. ;

Siebert, Reiner .

JOURNAL OF MOLECULAR DIAGNOSTICS, 2006, 8 (02) :141-151

← 1 2 →