Analysis of the humoral immune response to immunoselected phage-displayed peptides by a microarray-based method

被引：20

作者：

Cekaite, L ^{[1
]}

Haug, O

Myklebost, O

Aldrin, M

Ostenstad, B

Holden, M

Frigessi, A

Hovig, E

Sioud, M

机构：

[1] Norwegian Radium Hosp, Inst Canc Res, Dept Tumor Biol, N-0310 Oslo, Norway

[2] Norwegian Comp Ctr, Oslo, Norway

[3] Univ Oslo, Dept Mol Biosci, Oslo, Norway

[4] Ullevaal Univ Hosp, Dept Oncol, Oslo, Norway

[5] Univ Oslo, Sect Med Stat, Oslo, Norway

[6] Norwegian Radium Hosp, Dept Immunol, Mol Med Grp, Canc Res Inst, Oslo, Norway

来源：

PROTEOMICS | 2004年 / 4卷 / 09期

关键词：

phage display; protein microarray; regression; targeting peptides;

D O I：

10.1002/pmic.200300768

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a novel approach for high-throughput analysis of the immune response in cancer patients using phage-based microarray technology. The recombinant phages used for fabricating phage arrays were initially selected via the use of random peptide phage libraries and breast cancer patient serum antibodies. The peptides displayed by the phages retained their ability to be recognized by serum antibodies after immobilization. The recombinant phage microarrays were screened against either breast cancer or healthy donor serum antibodies. A model-based statistical method is proposed to estimate significant differences in serum antibody reactivity between patients and normals. A significant tumor effect was found with most of the selected phage-displayed peptides, suggesting that recombinant phage Microarrays can serve as a tool in monitoring humoral responses towards phage-displayed peptides.

引用

页码：2572 / 2582

页数：11

共 29 条

[11]

Jihoon Yang, 1999, Intelligent Data Analysis, V3, P55, DOI 10.1016/S1088-467X(99)00005-0

[12] In vitro protein microarrays for detecting protein-protein interactions:: Application of a new method for fluorescence labeling of proteins [J].

Kawahashi, Y ;

Doi, N ;

Takashima, H ;

Tsuda, C ;

Oishi, Y ;

Oyama, R ;

Yonezawa, M ;

Miyamoto-Sato, E ;

Yanagawa, H .

PROTEOMICS, 2003, 3 (07) :1236-1243

[13] Solid supports for microarray immunoassays [J].

Kusnezow, W ;

Hoheisel, JD .

JOURNAL OF MOLECULAR RECOGNITION, 2003, 16 (04) :165-176

[14]

Kusnezow W, 2002, BIOTECHNIQUES, P14

[15]

MacBeath G, 2000, SCIENCE, V289, P1760

[16] Antibody microarray profiling of human prostate cancer sera: Antibody screening and identification of potential biomarkers [J].

Miller, JC ;

Zhou, HP ;

Kwekel, J ;

Cavallo, R ;

Burke, J ;

Butler, EB ;

Teh, BS ;

Haab, BB .

PROTEOMICS, 2003, 3 (01) :56-63

[17] Quantitative assessment of peptide sequence diversity in M13 combinatorial peptide phage display libraries [J].

Rodi, DJ ;

Soares, AS ;

Makowski, L .

JOURNAL OF MOLECULAR BIOLOGY, 2002, 322 (05) :1039-1052

[18] HUMAN NEOPLASMS ELICIT MULTIPLE SPECIFIC IMMUNE-RESPONSES IN THE AUTOLOGOUS HOST [J].

SAHIN, U ;

TURECI, O ;

SCHMITT, H ;

COCHLOVIUS, B ;

JOHANNES, T ;

SCHMITS, R ;

STENNER, F ;

LUO, GR ;

SCHOBERT, I ;

PFREUNDSCHUH, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (25) :11810-11813

[19] Selection of ligands for polyclonal antibodies from random peptide libraries: Potential identification of (auto)antigens that may trigger B and T cell responses in autoimmune diseases [J].

Sioud, M ;

Forre, O ;

Dybwad, A .

CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 1996, 79 (02) :105-114

[20]

SIOUD M, 1994, CLIN EXP IMMUNOL, V98, P520

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