Coronavirus reverse genetic systems: Infectious clones and replicons

被引：97

作者：

Almazan, Fernando ^{[1
]}

Sola, Isabel ^{[1
]}

Zuniga, Sonia ^{[1
]}

Marquez-Jurado, Silvia ^{[1
]}

Morales, Lucia ^{[1
]}

Becares, Martina ^{[1
]}

Enjuanes, Luis ^{[1
]}

机构：

[1] CSIC, CNB, Dept Mol & Cell Biol, Madrid 28049, Spain

来源：

VIRUS RESEARCH | 2014年 / 189卷

关键词：

Coronavirus; Reverse genetics; Infectious clones; Replicons; RESPIRATORY SYNDROME CORONAVIRUS; MOUSE HEPATITIS-VIRUS; TRANSMISSIBLE GASTROENTERITIS CORONAVIRUS; TRANSIENT DOMINANT SELECTION; OPEN READING FRAME; BACTERIAL ARTIFICIAL CHROMOSOME; IN-VITRO; VACCINIA VIRUS; ESCHERICHIA-COLI; BRONCHITIS VIRUS;

D O I：

10.1016/j.virusres.2014.05.026

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Coronaviruses (CoVs) infect humans and many animal species, and are associated with respiratory, enteric, hepatic, and central nervous system diseases. The large size of the CoV genome and the instability of some Coy replicase gene sequences during its propagation in bacteria, represent serious obstacles for the development of reverse genetic systems similar to those used for smaller positive sense RNA viruses. To overcome these limitations, several alternatives to more conventional plasmid-based approaches have been established in the last 13 years. In this report, we briefly review and discuss the different reverse genetic systems developed for CoVs, paying special attention to the severe acute respiratory syndrome Coy (SARS-CoV). (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：262 / 270

页数：9

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