Nanoscale Metal-Organic Frameworks for Real-Time Intracellular pH Sensing in Live Cells

被引：255

作者：

He, Chunbai ^{[1
]}

Lu, Kuangda ^{[1
]}

Lin, Wenbin ^{[1
]}

机构：

[1] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2014年 / 136卷 / 35期

关键词：

DRUG-DELIVERY; LIVING CELLS; NANOPARTICLES; NANOSENSORS; ADSORPTION; HYDROGEN; RELEASE; OXYGEN;

D O I：

10.1021/ja507333c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Real-time measurement of intracellular pH in live cells is of great importance for understanding physiological/pathological processes and developing intracellular drug delivery systems. We report here the first use of nanoscale metal-organic frameworks (NMOFs) for intracellular pH sensing in live cells. Fluorescein isothiocyanate (FITC) was covalently conjugated to a UiO NMOF to afford F-UiO NMOFs with exceptionally high FITC loadings, efficient fluorescence, and excellent ratiometric pH-sensing properties. Upon rapid and efficient endocytosis, F-UiO remained structurally intact inside endosomes. Live cell imaging studies revealed endo- and exocytosis of F-UiO and endosome acidification in real time. Fluorescently labeled NMOFs thus represent a new class of nanosensors for intracellular pH sensing and provide an excellent tool for studying NMOF-cell interactions.

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页码：12253 / 12256

页数：4

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