Evaluation of some cereals, plants and tubers through protein composition

被引：17

作者：

Gorinstein, S ^{[1
]}

Jaramillo, NO

Medina, OJ

Rogriques, WA

Tosello, GA

Paredes-Lopez, O

机构：

[1] Hebrew Univ Jerusalem, Hadassah Med Sch, Sch Pharm, Dept Med Chem, IL-91120 Jerusalem, Israel

[2] Univ Antioqua, Dept Biol, Medellin, Colombia

[3] Univ Pedag & Tecnol Colombia, Fac Sci, Dept Food Chem, Tunja, Boyaca, Colombia

[4] Univ Sao Paulo, Inst Genet, BR-13400 Sao Paulo, Brazil

[5] IPN, Ctr Invest & Estudios Avanzados, Dept Biotecnol & Bioquim, Irapuato 36500, Gto, Mexico

来源：

JOURNAL OF PROTEIN CHEMISTRY | 1999年 / 18卷 / 06期

关键词：

plants; composition; proteins; electrophoresis;

D O I：

10.1023/A:1020610424625

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Wild and cultivated maize, sorghum, rice, amaranth, soybean, and cassava were screened for variability in seed storage proteins. Total seed proteins, albumin (Alb-1 and Alb-2), globulin, alcohol-soluble (A1 and A2), and glutelin (G1 and G2) fractions were investigated by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The comparison was done by the obtained protein patterns and their relative amounts. Using quantitative analysis of the protein composition and the electrophoretic patterns, the relationships between total proteins and amount of individual proteins were determined. Electrophoretic patterns of extracted proteins from investigated samples showed that the main protein subunits were concentrated between 10 and 45 kDa. Variation was found in major fractions and minor bands. Electrophoretic patterns of the protein fractions are directly related to the genetic background of the protein and can be identified and used to certify the genetic makeup of wild, cultivated, or newly derived cereals and plants.

引用

页码：687 / 693

页数：7

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