Validation of an appropriate reference gene for normalization of reverse transcription-quantitative polymerase chain reaction data from rectal cancer biopsies

被引：8

作者：

Ho-Pun-Cheung, Alexandre ^{[1
,2
,3
]}

Bascoul-Mollevi, Caroline ^{[4
]}

Assenat, Eric ^{[2
,5
]}

Bibeau, Frederic ^{[6
]}

Boissiere-Michot, Florence ^{[6
]}

Cellier, Dominic ^{[3
]}

Ychou, Marc ^{[2
,5
]}

Lopez-Crapez, Evelyne ^{[1
]}

机构：

[1] Val dAurelle Canc Inst, Dept Biol, F-34298 Montpellier, France

[2] Val dAurelle Canc Inst, INSERM, U896, F-34298 Montpellier, France

[3] Merck Sante, F-69008 Lyon, France

[4] Val dAurelle Canc Inst, Dept Biostat, F-34298 Montpellier, France

[5] Val dAurelle Canc Inst, Dept Med & Digest Oncol, F-34298 Montpellier, France

[6] Val dAurelle Canc Inst, Dept Pathol, F-34298 Montpellier, France

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 388卷 / 02期

关键词：

RT-PCR; Normalization; Reference gene; Rectal cancer; Quantification; TIME RT-PCR; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; EXPRESSION; VARIABILITY; TISSUES;

D O I：

10.1016/j.ab.2009.03.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gene expression quantification using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) requires data normalization using an invariable reference gene. Here we assessed the stability of 15 housekeeping genes in 31 tumor and normal rectal samples to validate a reliable reference gene for rectal cancer studies. Our data show that 18S and 28S RNA are stably expressed in all samples. Moreover, when used for normalization, 18S, but not 28S. greatly reduced unspecific variations of gene expression due to RNA degradation. These results demonstrate that 18S is an appropriate reference gene for normalization of RT-qPCR data from rectal cancer samples. (c) 2009 Elsevier Inc. All rights reserved.

引用

页码：348 / 350

页数：3

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