The murine DNA-PKcs gene consists of 86 exons dispersed in more then 250 kb

被引:18
作者
Fujimori, A [1 ]
Araki, R [1 ]
Fukumura, R [1 ]
Saito, T [1 ]
Mori, M [1 ]
Mita, K [1 ]
Tatsumi, K [1 ]
Abe, M [1 ]
机构
[1] NATL INST RADIOL SCI,DIV BIOL & ONCOL,INAGE KU,CHIBA 263,JAPAN
关键词
D O I
10.1006/geno.1997.4919
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The DNA-PKcs gene encodes the 465-kDa catalytic subunit of DNA-dependent protein kinase (DNA-PK), which associates with heterodimeric autoantigens Ku70 and Ku80 and exhibits protein kinase activity depending on DNA double-strand breaks. The gene is also responsible for the aberration in severe combined immune deficiency (SCID) mice, which exhibit a high sensitivity to ionizing radiation and abnormal DNA rearrangement of immunoglobulin and T cell receptor genes. There is further evidence that DNA-PKcs phosphorylates various proteins involved in DNA replication, transcription, repair, and recombination. Nevertheless the structure/function relationship in this huge molecule is virtually unknown. We determined the exons and introns of the murine DNA-PKcs gene by the long-distance polymerase chain reaction method. The murine DNA-PKcs gene consists of 86 exons distributed in a region of more than 250 kb. The average size of the exons is 140 bp., All the splicing sites conform to the GT/AG rule. The SCID mutation site (Tyr4046) has been identified in exon 85. The genomic structure of the DNA-PKcs gene provides clues for the study of various functional domains in this macromolecule. (C) 1997 Academic Press.
引用
收藏
页码:194 / 199
页数:6
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