Transglutaminase-catalyzed grafting collagen on chitosan and its characterization

被引：36

作者：

Fan, Lihong ^{[1
]}

Wu, Huan ^{[1
]}

Zhou, Xiaoyu ^{[1
]}

Peng, Min ^{[1
]}

Tong, Jun ^{[1
]}

Xie, Weiguo ^{[2
]}

Liu, Shuhua ^{[2
]}

机构：

[1] Wuhan Univ Technol, Coll Chem Engn, Wuhan 430070, Peoples R China

[2] Third Hosp Wuhan, Wuhan 430060, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2014年 / 105卷

基金：

中国国家自然科学基金;

关键词：

Collagen; Chitosan; Microbial transglutaminase; Antioxidant activity; Cell viability; SKIN; GELATIN; PROLIFERATION; CYTOTOXICITY; CARTILAGE; PEPTIDES; MATRICES; ENZYMES;

D O I：

10.1016/j.carbpol.2014.01.065

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Collagen grafted chitosan was prepared with microbial transglutaminase (MTGase) as biocatalyst which showed high efficiency, selectivity, mild reaction condition and environmental friendliness. The reaction conditions that influenced the degree of substitution (DS) were optimized, which included the reaction time, the reaction temperature, the mass ratio of collagen to chitosan and the mass ratio of MTGase to chitosan. In this study, the water-solubility collagen-chitosan could serve not only to reduce the loss of moisture but also to absorb the moisture. And the moisture absorption and moisture retention abilities were closely related to the DS values. In addition, in vitro antioxidant activity was evaluated in terms of DS values and concentration. Furthermore, L929 mouse fibroblasts were cultured with collagen-chitosan, and methylthiazol tetrazolium (MU) assay exhibited that collagen-chitosan with DS of 0.660 displayed pronounced cell viability at 2.5 mg/ml. Therefore, the water-soluble collagen-chitosan showed the potentiality to repair skin in cosmetic, biomedical and pharmaceutical fields. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：253 / 259

页数：7

共 33 条

[1]

Formulation and characterization of silk sericin-PVA scaffold crosslinked with genipin [J].