Study on the three-dimensional proliferation of rabbit articular cartilage-derived chondrocytes on polyhydroxyalkanoate scaffolds

被引:184
作者
Deng, Y
Zhao, K
Zhang, XF
Hu, P
Chen, GQ [1 ]
机构
[1] Tsing Hua Univ, Dept Biol Sci & Biotechnol, Microbiol Lab, Beijing 100084, Peoples R China
[2] Tsing Hua Univ, Dept Chem Engn, Beijing 100084, Peoples R China
基金
中国国家自然科学基金;
关键词
polyhydroxyalkanoates; PHB; biomaterial; tissue engineering; chondrocytes;
D O I
10.1016/S0142-9612(02)00136-9
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Polymer scaffold systems consisting of poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx)/polyhydroxybutyrate (PHB) (PHBHHx/PHB) were investigated for possible application as a matrix for the three-dimensional growth of chondrocyte culture. Blend polymers of PHBHHx/PHB were fabricated into three-dimensional porous scaffolds by the salt-leaching method. Chondrocytes isolated from rabbit articular cartilage (RAC) were seeded on the scaffolds and incubated over 28 days, with change of the culture medium every 4 days. PHB scaffold was taken as a control. Methylthiazol tetrazolium (MTT) (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltertra-zolium bromide) assay was used to quantitatively examine the proliferation of chondrocytes. Results showed that chondrocytes proliferated better on the PHBHHx/PHB scaffolds than on PHB one. The maximal cell densities were all observed after 7 days of incubation. As for the blend polymers, cells grew better on scaffolds consisting of PHBHHx/PHB in ratios of 2:1 and 1:2 than they did on PHBHHx/PHB of 1:1. Scanning electron microscopy (SEM) also showed that large quantities of chondrocytes grew initially on the surface of the scaffold. After 7 days, they further grew into the open pores of the blend polymer scaffolds. Morphologically, cells found on the surface of the scaffold exhibited a flat appearance and slowly form confluent cell multilayers starting from 14 to 28 days of the growth. In contrast, cells showed rounded morphology, formed aggregates and islets inside the scaffolds. In addition, chondrocytes proliferated on the scaffold and preserved their phenotype for up to 28 days. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:4049 / 4056
页数:8
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