CD40-Independent Engagement of Mammalian hsp70 by Antigen-Presenting Cells

被引：13

作者：

Binder, Robert J. ^{[1
]}

机构：

[1] Univ Pittsburgh, Dept Immunol, Pittsburgh, PA 15261 USA

来源：

JOURNAL OF IMMUNOLOGY | 2009年 / 182卷 / 11期

关键词：

HEAT-SHOCK PROTEINS; CYTOTOXIC T-LYMPHOCYTES; DENDRITIC CELLS; IN-VIVO; HEAT-SHOCK-PROTEIN-70; CD40; PEPTIDES; IMMUNITY; RECEPTOR; GENERATION;

D O I：

10.4049/jimmunol.0900026

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

CD40 has been suggested previously to be a receptor for mammalian murine hsc73 (hsp70). We have examined, in vitro and in vivo, the role of CD40 in the interaction of murine dendritic cells and macrophages with hsp70, using several independent parameters including cell surface binding, translocation of NF-kappa B, stimulation of release of TNF-alpha, representation of hsp70-chaperoned peptides, and priming of CD8(+) T cells. The various consequences of hsp70-APC interaction were compared between CD40(+/+) and CD40(-/-) mice and were found to be identical in kinetics and magnitude. These data strongly indicate that all known effects of mammalian hsp70 on APCs are mediated in a CD40-independent manner. In light of the earlier demonstration that mycobacterial hsp70 binds murine CD40 and stimulates the APCs through it, our data indicate that CD40 can discriminate between self and mycobacterial hsp70 and is thus a receptor for patterns associated with microbial pathogens. The Journal of Immunology, 2009, 182: 6844-6850.

引用

页码：6844 / 6850

页数：7

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