Relation between brain tissue pO2 and dopamine synthesis of basal ganglia -: A 18FDOPA-PET study in newborn piglets

被引:10
作者
Bauer, R [1 ]
Brust, P
Walter, B
Vorwieger, G
Bergmann, R
Füchtner, F
Steinbach, J
El-Hallag, E
Fritz, A
Johannsen, B
Zwiener, U
机构
[1] Klinikum Friedrich Schiller Univ, Inst Pathophysiol, D-07740 Jena, Germany
[2] Forschungszentrum Rossendorf EV, Inst Bioanorgan & Radiopharmazeut Chem, Dresden, Germany
关键词
brain tissue pO(2); cerebral blood flow; CMRO2; colored microspheres; DOPA decarboxylase activity; positron emission tomography;
D O I
10.1515/JPM.2000.008
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Perinatal hypoxic-ischemic cerebral injury is a major determinant of neurologic morbidity and mortality in the neonatal period and later in childhood. There is evidence that the dopaminergic system is sensitive to oxygen deprivation. However, the respective enzyme activities have yet not been measured in the living neonatal brain. In this study, we have used F-18-labelled 6-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) together with positron emission tomography (PET) to estimate the activity of the aromatic amino acid decarboxylase (AADC), the ultimate enzyme in the synthesis of dopamine, in the brain of newborn piglets under normoxic and moderate asphyxial conditions. The Study was performed on 8 newborn piglets (2-5 days old). In each piglet PET studies were performed under control conditions and during 2-hour asphyxia. Simultaneously, brain tissue pO(2) was recorded, cerebral blood flow (CBF was measured with colored microspheres and cerebral metabolic rate of oxygen (CMRO2) was determined. Asphyxia was induced by lowering the inspired fraction of oxygen from 0.35 to 0.10 and adding about 6 % CO2 to the inspired gas. Asphyxia elicited a more than 3-fold increase of the CBF (p < 0.01) so that CMRO2 remained unchanged throughout the asphyxial period. Despite this, brain tissue pO(2) was reduced from 19 +/- 4 mm Hg to 6 +/- 3 mm Mg ( p < 0.01). Blood-brain transfer of FDOPA as well as permeability-surface area product (PS) from striatum were unchanged. Striatal synthesis rate of fluoro-dopamine from FDOPA (k(3)) was, however, significantly increased (p < 0.01). This increase of the AADC activity is associated with reduced brain tissue pO(2). Asphyxia-induced CBF increase impedes an alteration of brain oxidative metabolism.
引用
收藏
页码:54 / 60
页数:7
相关论文
共 35 条
[1]   EFFECTS OF TRANSIENT FOREBRAIN ISCHEMIA AND REPERFUSION ON FUNCTION OF DOPAMINERGIC-NEURONS AND DOPAMINE REUPTAKE INVIVO IN RAT STRIATUM [J].
AKIYAMA, Y ;
ITO, A ;
KOSHIMURA, K ;
OHUE, T ;
YAMAGATA, S ;
MIWA, S ;
KIKUCHI, H .
BRAIN RESEARCH, 1991, 561 (01) :120-127
[2]   Regional distribution of cerebral blood volume and cerebral blood flow in newborn piglets - effect of hypoxia/hypercapnia [J].
Bauer, R ;
Bergmann, R ;
Walter, B ;
Brust, P ;
Zwiener, U ;
Johannsen, B .
DEVELOPMENTAL BRAIN RESEARCH, 1999, 112 (01) :89-98
[3]   Simultaneous measurement of [18F]FDOPA metabolism and cerebral blood flow in newborn piglets [J].
Brust, P ;
Bauer, R ;
Walter, B ;
Bergmann, R ;
Fuchtner, F ;
Vorwieger, G ;
Steinbach, J ;
Johannsen, B ;
Zwiener, U .
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, 1998, 16 (05) :353-364
[4]   AGE-RELATED CARDIOVASCULAR EFFECTS OF CATECHOLAMINES IN ANESTHETIZED PIGLETS [J].
BUCKLEY, NM ;
GOOTMAN, PM ;
YELLIN, EL ;
BRAZEAU, P .
CIRCULATION RESEARCH, 1979, 45 (02) :282-292
[5]   STRIATAL PROTECTION INDUCED BY LESIONING THE SUBSTANTIA-NIGRA OF RATS SUBJECTED TO FOCAL ISCHEMIA [J].
BUISSON, A ;
CALLEBERT, J ;
MATHIEU, E ;
PLOTKINE, M ;
BOULU, RG .
JOURNAL OF NEUROCHEMISTRY, 1992, 59 (03) :1153-1157
[6]   HYPERTHERMIA INCREASES CEREBRAL METABOLIC-RATE AND BLOOD-FLOW IN NEONATAL PIGS [J].
BUSIJA, DW ;
LEFFLER, CW ;
POURCYROUS, M .
AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 255 (02) :H343-H346
[7]   CATHETERIZATION OF THE SAGITTAL SINUS IN THE NEONATAL PIG [J].
FLECKNELL, PA ;
JOHN, M .
LABORATORY ANIMALS, 1983, 17 (01) :32-34
[8]   EFFECT OF DECREASED OXYGEN ON INVITRO RELEASE OF ENDOGENOUS 3,4-DIHYDROXYPHENYLETHYLAMINE FROM MOUSE STRIATUM [J].
FREEMAN, GB ;
GIBSON, GE .
JOURNAL OF NEUROCHEMISTRY, 1986, 47 (06) :1924-1931
[9]   REGIONAL GLUCOSE-UTILIZATION AND BLOOD-FLOW FOLLOWING GRADED FOREBRAIN ISCHEMIA IN THE RAT - CORRELATION WITH NEUROPATHOLOGY [J].
GINSBERG, MD ;
GRAHAM, DI ;
BUSTO, R .
ANNALS OF NEUROLOGY, 1985, 18 (04) :470-481
[10]  
Gjedde A., 1990, QUANTITATIVE IMAGING