A collection of 1814 human chromosome 7-specific STSs

被引：16

作者：

Bouffard, GG

Iyer, LM

Idol, JR

Braden, VV

Cunningham, AF

Weintraub, LA

MohrTidwell, RM

Peluso, DC

Fulton, RS

Leckie, MP

Green, ED

机构：

[1] NIH, NATL CTR HUMAN GENOME RES, GENOME TECHNOL BRANCH, BETHESDA, MD 20892 USA

[2] WASHINGTON UNIV, SCH MED, DEPT GENET, ST LOUIS, MO 63110 USA

来源：

GENOME RESEARCH | 1997年 / 7卷 / 01期

关键词：

D O I：

10.1101/gr.7.1.59

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An established goal of the ongoing Human Genome Project is the development. and mapping of sequence-tagged sites (STSs) every 100 kb, on average, across all human chromosomes. En route to constructing such a physical map of human chromosome 7, we have generated 1814 chromosome 7-specific STSs. The corresponding PCR assays were designed by the use of DNA sequence determined in our laboratory (79%) or generated elsewhere (21%) and were demonstrated to be suitable for screening yeast artificial chromosome (YAC) libraries. This collection provides the requisite landmarks for constructing a physical map of chromosome 7 at <100-kb average spacing of STSs.

引用

页码：59 / 64

页数：6

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