Chemopreventive activity of apple extract following medium-term oral carcinogenesis assay induced by 4-nitroquinoline-1-oxide

被引：16

作者：

Pidone Ribeiro, Flavia Andressa ^{[1
]}

Gomes de Moura, Carolina Foot ^{[2
]}

Boiago Gollucke, Andrea Pitelli ^{[3
]}

Ferreira, Monica Siqueira ^{[4
]}

Catharino, Rodrigo Ramos ^{[4
]}

Aguiar, Odair, Jr. ^{[1
]}

Spadari, Regina Celia ^{[1
]}

Barbisan, Luis Fernando ^{[5
]}

Ribeiro, Daniel Araki ^{[1
,3
]}

机构：

[1] Univ Fed Sao Paulo, UNIFESP, Dept Biociencias, BR-11060001 Santos, SP, Brazil

[2] Univ Fed Sao Paulo, UNIFESP, Dept Pathol, BR-11060001 Santos, SP, Brazil

[3] Catholic Santos Univ, Hexalab, Santos, SP, Brazil

[4] Univ Estadual Campinas, Med & Expt Surg Nucleus, INNOVARE Biomarkers Lab, Campinas, SP, Brazil

[5] Univ Estadual Paulista, Inst Biosci, Dept Morphol, Botucatu, SP, Brazil

来源：

ARCHIVES OF ORAL BIOLOGY | 2014年 / 59卷 / 08期

关键词：

Apple extract; 4-Nitroquinoline-1-oxide; Oral cancer; Inflammation; Apoptosis; CANCER-RELATED INFLAMMATION; MITOCHONDRIAL PATHWAY; COLON CARCINOGENESIS; MOUSE MODEL; APOPTOSIS; CELLS; RATS; CARCINOMA; PROCYANIDINS; EXPRESSION;

D O I：

10.1016/j.archoralbio.2014.04.013

中图分类号：

R78 [口腔科学];

学科分类号：

100302 [口腔临床医学];

摘要：

Objective: The aim of this study was to evaluate the chemopreventive activity of an apple extract following medium-term oral carcinogenesis assay induced by 4-nitroquinoline-1-oxide (4NQO). Methods: A total of 30 male Wistar rats were distributed into five groups as follows (n = 6 per group): Group 1, negative control group (non-treated group); Group 2, received 4NQO during 8 weeks in drinking water and treated with apple extract at 1% by gavage between the first and fourth weeks daily (initiation phase); Group 3, received 4NQO for 8 weeks in drinking water and treated with apple extract by gavage at 1% between the fifth and eighth weeks daily (promotion phase); Group 4, received apple extract at 1% by gavage for 8 consecutive weeks only; and Group 5, received 4NQO for 8 weeks in drinking water daily. Results: Histopathological analysis revealed decreased hyperplasic lesions in Group 2 when compared with Group 5. Likewise, decreased dysplastic lesions in Group 3 were observed when compared with Group 5. In Groups 2 and 3, decreased COX-2 and TNF-alpha gene expressions were observed when compared with Group 5. Cytochrome c and caspase 3 levels increased in Groups 2 and 3 when compared with Group 5. Conclusion: In conclusion, our results demonstrate that apple extract suppresses rat tongue carcinogenesis as a result of anti-inflammatory activity and apoptosis through the intrinsic mitochondrial pathway. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：815 / 821

页数：7

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