Gastrodia elata prevents rat pheochromocytoma cells from serum-deprived apoptosis:: the role of the MAPK family

被引：43

作者：

Huang, NK ^{[1
]}

Lin, YL ^{[1
]}

Cheng, JJ ^{[1
]}

Lai, WL ^{[1
]}

机构：

[1] Natl Res Inst Chinese Med, Taipei 112, Taiwan

来源：

LIFE SCIENCES | 2004年 / 75卷 / 13期

关键词：

G; elata; PC12; cells; serum deprivation; apoptosis; MAPK;

D O I：

10.1016/j.lfs.2004.05.008

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

Gastrodia elata (G. elata) is a traditional Chinese herbal medicine for treating headaches, dizziness, tetanus, and epilepsy. In this study, differential methanol (MeOH) extracts of G. elata were found to prevent serum-deprived rat pheochromocytoma (PC 12) cell apoptosis by the MTT assay and Hoechst staining. A serine/threonine kinase inhibitor attenuated this protection. G. elata resulted in phosphorylation and dephosphorylation of ERK1/2 and JNK1/2-p38 MAPKs (members of the serine/threonine kinase family), respectively, as revealed by Western blot analysis. An upstream ERK inhibitor attenuated G. elata-induced ERK phosphorylation but not protective effect. Although JNK and p38 inhibitors attenuated their related enzyme activities during serum deprivation, only INK inhibitor prevented serum-deprived apoptosis. Thus, G. elata prevents serum-deprived apoptosis through activation of the serine/threonine kinase-dependent pathway and suppression of INK activity. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：1649 / 1657

页数：9

共 23 条

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MEK1 protein kinase inhibition protects against damage resulting from focal cerebral ischemia [J].