Regulation of copper/zinc and manganese superoxide dismutase by UVB irradiation, oxidative stress and cytokines

被引:54
作者
Isoherranen, K
Peltola, V
Laurikainen, L
Punnonen, J
Laihia, J
Ahotupa, M
Punnonen, K
机构
[1] UNIV TURKU,MED RES LAB,FIN-20520 TURKU,FINLAND
[2] UNIV TURKU,DEPT PHYSIOL,FIN-20520 TURKU,FINLAND
[3] UNIV TURKU,DEPT DERMATOL,FIN-20520 TURKU,FINLAND
[4] UNIV TURKU,DEPT MED MICROBIOL,FIN-20520 TURKU,FINLAND
[5] UNIV TURKU,MCA LAB,FIN-20520 TURKU,FINLAND
[6] KUOPIO UNIV HOSP,DEPT CLIN CHEM,FIN-70210 KUOPIO,FINLAND
基金
芬兰科学院;
关键词
superoxide dismutase; UVB irradiation; oxidative stress; carcinogenesis;
D O I
10.1016/S1011-1344(97)00071-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have examined the effects of UVB irradiation, oxidative stress and cytokines on the antioxidant enzymes copper/zinc and manganese superoxide dismutase (CuZnSOD and MnSOD) in HeLa cells. A single dose of UVB irradiation regulated dose-dependently the expression of the 4 kb transcript of MnSOD although it did not have any significant effect on MnSOD enzymatic activity, In contrast, UVB irradiation reduced both the enzymatic activity and the expression of the 0.7 and 0.9 kb mRNA transcripts of CuZnSOD. The cytokines TNF-alpha (1 ng ml(-1) and 10 ng ml(-1)) and IL-6 (100 U ml(-1)) induced MnSOD activity, and TNF-alpha also upregulated MnSOD mRNA expression, Interestingly, genistein, a soy isoflavone and a tyrosine kinase inhibitor, was able to inhibit the induction of Mn-SOD activity and mRNA expression by TNF-alpha. Enzymatic CuZnSOD activity was depressed by a high dose of H2O2 while IL-6 or TNF-alpha had no effect on CuZnSOD activity. Our results demonstrate that, in addition to enzyme activity level, UVB irradiation can regulate the superoxide dismutases at the mRNA level. We also suggest that UVB irradiation, oxidative stress and cytokines regulate differentially CuZnSOD and MnSOD, and that the activities and expression of these antioxidant enzymes are controlled by distinct mechanisms. (C) 1997 Elsevier Science S.A.
引用
收藏
页码:288 / 293
页数:6
相关论文
共 47 条
[31]  
PUNNONEN K, 1991, ACTA DERM-VENEREOL, V71, P239
[32]   Effects of a single exposure to UVB radiation on the activities and protein levels of copper-zinc and manganese superoxide dismutase in cultured human keratinocytes [J].
Sasaki, H ;
Akamatsu, H ;
Horio, T .
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1997, 65 (04) :707-713
[33]   EFFECT OF UV IRRADIATION ON EPIDERMAL-CELL CYTOKINE PRODUCTION [J].
SCHWARZ, T ;
LUGER, TA .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1989, 4 (01) :1-13
[34]   ANTIOXIDANT DEFENSE-MECHANISMS IN MURINE EPIDERMIS AND DERMIS AND THEIR RESPONSES TO ULTRAVIOLET-LIGHT [J].
SHINDO, Y ;
WITT, E ;
PACKER, L .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1993, 100 (03) :260-265
[35]  
SHULL S, 1991, J BIOL CHEM, V266, P24398
[36]   Oxidative stress, caloric restriction, and aging [J].
Sohal, RS ;
Weindruch, R .
SCIENCE, 1996, 273 (5271) :59-63
[37]   LOWERED ANTIOXIDANT ENZYMES IN SPONTANEOUSLY TRANSFORMED EMBRYONIC MOUSE-LIVER CELLS IN CULTURE [J].
SUN, Y ;
OBERLEY, LW ;
OBERLEY, TD ;
ELWELL, JH ;
SIERRARIVERA, E .
CARCINOGENESIS, 1993, 14 (07) :1457-1463
[38]   HYBRIDIZATION OF DENATURED RNA AND SMALL DNA FRAGMENTS TRANSFERRED TO NITROCELLULOSE [J].
THOMAS, PS .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (09) :5201-5205
[39]   MOLECULAR-GENETICS OF SUPEROXIDE DISMUTASES [J].
TOUATI, D .
FREE RADICAL BIOLOGY AND MEDICINE, 1988, 5 (5-6) :393-402
[40]   ACUTE-PHASE INDUCTION OF MANGANESE SUPEROXIDE-DISMUTASE IN INTESTINAL EPITHELIAL-CELL LINES [J].
VALENTINE, JF ;
NICK, HS .
GASTROENTEROLOGY, 1992, 103 (03) :905-912