Quantum dots-based immunofluorescence technology for the quantitative determination of HER2 expression in breast cancer

被引：178

作者：

Chen, Chuang ^{[1
,2
,3
]}

Peng, Jun ^{[4
,5
]}

Xia, He-Shun ^{[6
]}

Yang, Gui-Fang ^{[7
]}

Wu, Qjong-Shui ^{[8
]}

Chen, Liang-Dong ^{[1
,2
,3
]}

Zeng, Li-Bo ^{[8
]}

Zhang, Zhi-Ling ^{[4
,5
]}

Pang, Dai-Wen ^{[1
,4
,5
]}

Li, Yan ^{[1
,2
,3
]}

机构：

[1] Wuhan Univ, Zhongnan Hosp, Dept Oncol, Wuhan 430071, Peoples R China

[2] Hubei Canc Clin Study Ctr, Wuhan 430071, Peoples R China

[3] Hubei Key Lab Tumor Biol Behav, Wuhan 430071, Peoples R China

[4] Wuhan Univ, State Key Lab Virol, Wuhan 430071, Peoples R China

[5] Wuhan Univ, Minist Educ, Key Lab Analyt Chem Biol & Med, Coll Chem & Mol Sci, Wuhan 430071, Peoples R China

[6] Hubei Canc Hosp, Dept Pathol, Wuhan 430079, Hubei, Peoples R China

[7] Wuhan Univ, Zhongnan Hosp, Dept Pathol, Wuhan 430071, Peoples R China

[8] Wuhan Univ, Elect Informat Sch, Sci Instrument Engn Res Ctr, Wuhan 430072, Peoples R China

来源：

BIOMATERIALS | 2009年 / 30卷 / 15期

基金：

中国国家自然科学基金;

关键词：

Quantum dots; Breast cancer; HER2; Immunohistochemistry; Fluorescence in situ hybridization; TIME; TRASTUZUMAB; BIOMARKERS; MORTALITY; ONCOGENE; THERAPY; PROBES; TUMORS;

D O I：

10.1016/j.biomaterials.2009.02.010

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

HER2 detection is important for breast cancer (BC) treatment and prognosis, but the detection methods currently used have some disadvantages. Quantum dots (QDs)-based probes provide a potentially important new method for HER2 detection in clinical practice. This potential is examined in this paper. A QDs HER2 probe kit and QDs image acquisition and analysis software were developed and applied to 94 clinical samples of BC. Compared to conventional immunohistochemistry techniques, this method provided a superior accurate and sensitive method for the detection of HER2 in clinical breast cancer diagnosis. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：2912 / 2918

页数：7

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