The biocompatibility of quantum dot probes used for the targeted imaging of hepatocellular carcinoma metastasis

被引：152

作者：

Chen, Liang-Dong ^{[1
]}

Liu, Jia ^{[2
]}

Yu, Xue-Feng ^{[3
]}

He, Man ^{[2
]}

Pei, Xiao-Feng ^{[1
]}

Tang, Zhao-You ^{[4
]}

Wang, Qu-Quan ^{[3
]}

Pang, Dai-Wen ^{[2
]}

Li, Yan ^{[1
]}

机构：

[1] Wuhan Univ, Zhongnan Hosp, Dept Oncol, Hubei Key Lab Tumor Biol Behav, Wuhan 430071, Peoples R China

[2] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430071, Peoples R China

[3] Wuhan Univ, Dept Phys, Wuhan 430071, Peoples R China

[4] Fudan Univ, Zhongshan Hosp, Liver Canc Inst, Shanghai 200032, Peoples R China

来源：

BIOMATERIALS | 2008年 / 29卷 / 31期

关键词：

Quantum dots; Hepatocellular carcinoma; Metastasis; Toxicity; Biocompatibility;

D O I：

10.1016/j.biomaterials.2008.07.025

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Semiconductor quantum dots (QDs) have several photo-physical advantages over organic dyes making them good markers in biomedical application. We used CdSe/ZnS QDs with maximum emission wavelength of 590 nm (QD590) linked to alpha-fetoprotein (AFP) monoclonal antibody (Ab) to detect AFP in cytoplasm of human hepatocellular carcinoma (HCC) cell line HCCLM6. For the in vivo studies, we used QD-AFP-Ab probes for targeted imaging of human HCC xenograft growing in nude mice by injecting them into the tail vein. In addition, the cytotoxicity in vitro, the acute toxicity in vivo, the hemodynamics and tissue distribution of these probes were also investigated. The results in vitro and in vivo indicate that our QD-based probes have good stability, specificity and biocompatibility for ultrasensitive fluorescence imaging of molecular targets in our liver cancer model system. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：4170 / 4176

页数：7

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