Engineering of functional tendon

被引:118
作者
Calve, S
Dennis, RG
Kosnik, PE
Baar, K
Grosh, K
Arruda, EM
机构
[1] Univ Michigan, Dept Mech Engn, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Program Macromol Sci & Engn, Ann Arbor, MI 48109 USA
[3] Univ Michigan, Inst Gerontol, Ann Arbor, MI 48109 USA
[4] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[5] Tissue Genesis, Honolulu, HI USA
来源
TISSUE ENGINEERING | 2004年 / 10卷 / 5-6期
关键词
D O I
10.1089/1076327041348464
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Surgical tendon repair is limited by the availability of viable tissue for transplantation. Because of its relatively avascular nature, tendon is a prime candidate for engineered tissue replacement. To address this problem, cells isolated from rat Achilles tendon were grown to confluence in culture and allowed to self-assemble into a cylinder between two anchor points. The resulting scaffold-free tissue was composed of aligned, small-diameter collagen fibrils, a large number of cells, and an excess of noncollagenous extracellular matrix; all characteristics of embryonic tendon. The stress-strain response of the constructs also resembles the nonlinear behavior of immature tendons, and the ultimate tensile strength is approximately equal to that of embryonic chick tendon, roughly 2 MPa. These physical and mechanical properties indicate that these constructs are the first viable tendons engineered in vitro, without the aid of artificial scaffolding.
引用
收藏
页码:755 / 761
页数:7
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