Gossypol induced apoptosis in the human promyelocytic leukemia cell line HL 60

被引：28

作者：

Balci, A ^{[1
]}

Sahin, FI ^{[1
]}

Ekmekci, A ^{[1
]}

机构：

[1] Gazi Univ, Fac Med, Dept Med Biol & Genet, TR-06510 Ankara, Turkey

来源：

TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE | 1999年 / 189卷 / 01期

关键词：

apoptosis; gossypol; protein kinase;

D O I：

10.1620/tjem.189.51

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

In human promyelocytic leukemia cell line HL60, apoptosis was induced by treatment with gossypol that is an inhibitor of protein kinase C. Gossypol acetic acid mas added to HL 60 cells at 50, 100, 180 and 200 mu M concentrations for six hours. Morphological features of apoptosis as well as internucleosomal DNA fragmentation mere evaluated by light microscope, agarose gel electrophoresis and spectrofluorometric quantitation. Our results indicated that with the effective concentrations of gossypol (50 and 100 mu M), apoptosis was induced in HL 60 cells.

引用

页码：51 / 57

页数：7

共 16 条

[1] APOPTOSIS AND DISEASE [J].

CARSON, DA ;

RIBEIRO, JM .

LANCET, 1993, 341 (8855) :1251-1254

[2] KEY MORPHOLOGICAL FEATURES OF APOPTOSIS MAY OCCUR IN THE ABSENCE OF INTERNUCLEOSOMAL DNA FRAGMENTATION [J].

COHEN, GM ;

SUN, XM ;

SNOWDEN, RT ;

DINSDALE, D ;

SKILLETER, DN .

BIOCHEMICAL JOURNAL, 1992, 286 :331-334

[3]

DIPASQUALE B, 1991, J IMMUNOL RES, V3, P149

[4] APOPTOSIS IN CANCER-THERAPY - CROSSING THE THRESHOLD [J].

FISHER, DE .

CELL, 1994, 78 (04) :539-542

[5] Modulation of protein kinase C activity and calcium-sensitive isoform expression in human myeloid leukemia cells by bryostatin 1: Relationship to differentiation and ara-C-induced apoptosis [J].

Grant, S ;

Turner, AJ ;

Freemerman, AJ ;

Wang, ZL ;

Kramer, L ;

Jarvis, WD .

EXPERIMENTAL CELL RESEARCH, 1996, 228 (01) :65-75

[6]

HALICKA HD, 1995, CANCER RES, V55, P444

[7]

Hunakova L, 1996, NEOPLASMA, V43, P291

[8] PROGRAMMED CELL-DEATH AND BCL-2 PROTECTION IN THE ABSENCE OF A NUCLEUS [J].

JACOBSON, MD ;

BURNE, JF ;

RAFF, MC .

EMBO JOURNAL, 1994, 13 (08) :1899-1910

[9]

JARVIS WD, 1994, CANCER RES, V54, P1707

[10] APOPTOSIS - BASIC BIOLOGICAL PHENOMENON WITH WIDE-RANGING IMPLICATIONS IN TISSUE KINETICS [J].

KERR, JFR ;

WYLLIE, AH ;

CURRIE, AR .

BRITISH JOURNAL OF CANCER, 1972, 26 (04) :239-+

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