The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells

被引：212

作者：

Simon-Plas, F ^{[1
]}

Elmayan, T ^{[1
]}

Blein, JP ^{[1
]}

机构：

[1] Univ Bourgogne, INRA, UMR692, Lab Phytopharm & Biochim Interact Cellulaires, F-21065 Dijon, France

来源：

PLANT JOURNAL | 2002年 / 31卷 / 02期

关键词：

active oxygen species; cryptogein; elicitation; NADPH oxidase; plasma membrane; tobacco;

D O I：

10.1046/j.1365-313X.2002.01342.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A cDNA encoding a protein, NtrbohD, located on the plasma membrane and homologue to the flavocytochrome of the neutrophil NADPH oxidase, was cloned in tobacco. The corresponding mRNA was accumulated when tobacco leaves and cells were treated with the fungal elicitor cryptogein. After elicitation with cryptogein, tobacco cells transformed with antisense constructs of NtrbohD showed the same extracellular alkalinization as the control, but no longer produced active oxygen species (AOS). This work represents the first demonstration of the function of a homologue of gp91(-phox) in AOS production in elicited tobacco cells.

引用

页码：137 / 147

页数：11

共 53 条

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