Evidence for a Ustilago maydis steroid 5α-reductase by functional expression in Arabidopsis det2-1 mutants

被引:6
作者
Basse, CW
Kerschbamer, C
Brustmann, M
Altmann, T
Kahmann, R
机构
[1] Univ Munich, Inst Genet & Mikrobiol, D-80638 Munich, Germany
[2] Max Planck Inst Mol Pflanzenphysiol, D-14476 Golm, Germany
关键词
D O I
10.1104/pp.001016
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We have identified a gene (udh1) in the basidiomycete Ustilago maydis that is induced during the parasitic interaction with its host plant maize (Zea mays). udh1 encodes a protein with high similarity to mammalian and plant 5alpha-steroid reductases. Udh1 differs from those of known 5alpha-steroid reductases by six additional domains, partially predicted to be membrane-spanning. A fusion protein of Udh1 and the green fluorescent protein provided evidence for endoplasmic reticulum localization in U. maydis. The function of the Udh1 protein was demonstrated by complementing Arabidopsis det2-1 mutants, which display a dwarf phenotype due to a mutation in the 5alpha-steroid reductase encoding DET2 gene. det2-1 mutant plants expressing either the udh1 or the DET2 gene controlled by the cauliflower mosaic virus 35S promoter differed from wild-type Columbia plants by accelerated stem growth, flower and seed development and a reduction in size and number of rosette leaves. The accelerated growth phenotype of udh1 transgenic plants was stably inherited and was favored under reduced light conditions. Truncation of the N-terminal 70 amino acids of the Udhl protein abolished the ability to restore growth in det2-1 plants. Our results demonstrate the existence of a 5alpha-steroid reductase encoding gene in fungi and suggest a common ancestor between fungal, plant, and mammalian proteins.
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页码:717 / 732
页数:16
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